Fatty acid profiling of Chlamydomonas reinhardtii under nitrogen deprivation
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James, Gabriel; Hocart, Charles; Hillier, Warwick; Chen, Hancai; Kordbacheh, Farzaneh; Price, Graeme (Dean); Djordjevic, Michael
Description
The Chlamydomonas reinhardtii starch-less mutant, BAF-J5, was found to store lipids up to 65% of dry cell weight when grown photoheterotrophically and subjected to nitrogen starvation. Fourier transform infrared spectroscopy was used as a high-throughput method for semi-quantitative measurements of protein, carbohydrate and lipid content. The fatty acids of wild-type and starch mutants were identified and quantified by gas chromatography mass spectrometry. C. reinhardtii starch mutants,...[Show more]
Collections | ANU Research Publications |
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Date published: | 2011 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/28793 |
Source: | Bioresource Technology |
DOI: | 10.1016/j.biortech.2010.11.051 |
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01_James_Fatty_acid_profiling_of__2011.pdf | 689.95 kB | Adobe PDF | ![]() |
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