James, Gabriel; Hocart, Charles; Hillier, Warwick; Chen, Hancai; Kordbacheh, Farzaneh; Price, Graeme (Dean); Djordjevic, Michael
The Chlamydomonas reinhardtii starch-less mutant, BAF-J5, was found to store lipids up to 65% of dry cell
weight when grown photoheterotrophically and subjected to nitrogen starvation. Fourier transform
infrared spectroscopy was used as a high-throughput method for semi-quantitative measurements of
protein, carbohydrate and lipid content. The fatty acids of wild-type and starch mutants were identified
and quantified by gas chromatography mass spectrometry. C. reinhardtii starch mutants,...[Show more]
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