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Transcriptome-wide analysis of pseudouridylation of mRNA and non-coding RNAs in Arabidopsis

Sharwood, Robert


Pseudouridine (Ψ) is widely distributed in mRNA and various non-coding RNAs in yeast and mammals, and the specificity of its distribution has been determined. However, knowledge about Ψs in the RNAs of plants, particularly in mRNA, is lacking. In this study, we performed genome-wide pseudouridine-sequencing in Arabidopsis and for the first time identified hundreds of Ψ sites in mRNA and multiple Ψ sites in non-coding RNAs. Many predicted and novel Ψ sites in rRNA and tRNA were detected. mRNA...[Show more]

CollectionsANU Research Publications
Date published: 2019
Type: Journal article
Source: Journal of Experimental Botany
DOI: 10.1093/jxb/erz273
Access Rights: Open Access


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