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Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: A multi-center evaluation

Gurumurthy, Channabasavaiah B.; O'Brien, Aidan; Quadros, Rolen M.; Adams Jr, John; Alcaide, Pilar; Ayabe, Shinya; Ballard, John; Batra, Surinder K.; Beauchamp, Marie-Claude; Becker, Kathleen A.; Bernas, Guillaume; Jing, Gao; Khin, Nay Chi; Lowe, Jenna; Ross, Nikki; Starrs, Lora; Burgio, Gaetan


Background CRISPR-Cas9 gene-editing technology has facilitated the generation of knockout mice, providing an alternative to cumbersome and time-consuming traditional embryonic stem cell-based methods. An earlier study reported up to 16% efficiency in generating conditional knockout (cKO or floxed) alleles by microinjection of 2 single guide RNAs (sgRNA) and 2 single-stranded oligonucleotides as donors (referred herein as “two-donor floxing” method). Results We re-evaluate the two-donor...[Show more]

CollectionsANU Research Publications
Date published: 2019
Type: Journal article
Source: Genome Biology
DOI: 10.1186/s13059-019-1776-2
Access Rights: Open Access


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