Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies
Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).
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|Source:||Angewandte Chemie International Edition|
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