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Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies

Wu, Peter; Ozawa, Kiyoshi; Lim, Siew P; Vasudevan, Subhash G.; Dixon, Nicholas Edward; Otting, Gottfried

Description

Quick and clean: Excellent protein yields in cell-free protein synthesis can be obtained by using polymerase chain reaction (PCR) amplified DNA templates, provided that the templates are designed for cyclization. This eliminates time-consuming cloning steps and allows one to go from complementary DNA to the protein NMR spectrum in 24 h. (Graph Presented).

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
URI: http://hdl.handle.net/1885/28139
Source: Angewandte Chemie International Edition
DOI: 10.1002/anie.200605237

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