Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene
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Qui, Min Ru; Jiang, Lele; Matthaei, Klaus; Schoenwaelder, Simone M; Kuffner, Tamara; Mangin, Pierre; Joseph, Joanne E; Low, Joyce; Connor, David; Valenzuela, Stella M; Curmi, Paul; Brown, Louise J; Mahaut-smith, Martyn; Jackson, Shaun P; Breit, Samuel N.
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Summary: CLIC1 belongs to a family of highly conserved and widely expressed intracellular chloride ion channel proteins existing in both soluble and membrane integrated forms. To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1FN) allele, followed by Clic1 knockout...[Show more]
dc.contributor.author | Qui, Min Ru | |
---|---|---|
dc.contributor.author | Jiang, Lele | |
dc.contributor.author | Matthaei, Klaus | |
dc.contributor.author | Schoenwaelder, Simone M | |
dc.contributor.author | Kuffner, Tamara | |
dc.contributor.author | Mangin, Pierre | |
dc.contributor.author | Joseph, Joanne E | |
dc.contributor.author | Low, Joyce | |
dc.contributor.author | Connor, David | |
dc.contributor.author | Valenzuela, Stella M | |
dc.contributor.author | Curmi, Paul | |
dc.contributor.author | Brown, Louise J | |
dc.contributor.author | Mahaut-smith, Martyn | |
dc.contributor.author | Jackson, Shaun P | |
dc.contributor.author | Breit, Samuel N. | |
dc.date.accessioned | 2015-12-07T22:54:17Z | |
dc.identifier.issn | 1526-954X | |
dc.identifier.uri | http://hdl.handle.net/1885/28116 | |
dc.description.abstract | Summary: CLIC1 belongs to a family of highly conserved and widely expressed intracellular chloride ion channel proteins existing in both soluble and membrane integrated forms. To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1FN) allele, followed by Clic1 knockout (Clic1-/-) mice by crossing Clic1FN allele with TNAPcre mice, resulting in germline gene deletion through Cre-mediated recombination. Mice heterozygous or homozygous for these alleles are viable and fertile and appear normal. However, Clic1-/- mice show a mild platelet dysfunction characterized by prolonged bleeding times and decreased platelet activation in response to adenosine diphosphate stimulation linked to P2Y12 receptor signaling. | |
dc.publisher | Wiley-Liss Inc | |
dc.source | Genesis | |
dc.subject | Keywords: adenosine diphosphate; chloride channel; chloride intracellular channel 1; cre recombinase; purinergic P2Y12 receptor; unclassified drug; animal experiment; article; bleeding time; gene deletion; gene mutation; gene targeting; heterozygosity; homozygote; Chloride intracellular channel family; CLIC1; Cre; Platelet | |
dc.title | Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 48 | |
dc.date.issued | 2010 | |
local.identifier.absfor | 100104 - Genetically Modified Animals | |
local.identifier.ariespublication | f2965xPUB56 | |
local.type.status | Published Version | |
local.contributor.affiliation | Qui, Min Ru, St. Vincent’s Hospital and University of New South Wales, | |
local.contributor.affiliation | Jiang, Lele, St. Vincent’s Hospital and University of New South Wales, | |
local.contributor.affiliation | Matthaei, Klaus, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Schoenwaelder, Simone M, Monash University | |
local.contributor.affiliation | Kuffner, Tamara, St. Vincent’s Hospital and University of New South Wales, | |
local.contributor.affiliation | Mangin, Pierre, Monash University | |
local.contributor.affiliation | Joseph, Joanne E, St Vincent's Hospital | |
local.contributor.affiliation | Low, Joyce, St Vincent's Hospital | |
local.contributor.affiliation | Connor, David, St Vincent's Hospital | |
local.contributor.affiliation | Valenzuela, Stella M, University of Technology Sydney | |
local.contributor.affiliation | Curmi, Paul, University of New South Wales | |
local.contributor.affiliation | Brown, Louise J, Macquarie University | |
local.contributor.affiliation | Mahaut-smith, Martyn, University of Leicester | |
local.contributor.affiliation | Jackson, Shaun P, Monash University | |
local.contributor.affiliation | Breit, Samuel N., St. Vincent's Hospital (University of New South Wales) | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 2 | |
local.bibliographicCitation.startpage | 127 | |
local.bibliographicCitation.lastpage | 136 | |
local.identifier.doi | 10.1002/dvg.20590 | |
dc.date.updated | 2016-02-24T08:30:30Z | |
local.identifier.scopusID | 2-s2.0-77249110323 | |
local.identifier.thomsonID | 000274719500008 | |
Collections | ANU Research Publications |
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