Measurement of dissociation constants of high-molecular weight protein-protein complexes by transferred 15 N-relaxation
The use of 15N-relaxation data for determination of the dissociation constant of a protein-protein complex is proposed for the situation where a 15N-labeled protein is bound to an unlabeled protein of high molecular weight, and the chemical exchange between bound and free protein is fast on the NMR time scale. The approach is shown to be suitable for estimating dissociation constants in the micromolar to millimolar range, using protein solutions at relatively low concentration. An example is...[Show more]
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|Source:||Journal of Biomolecular NMR|
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