Skip navigation
Skip navigation

Chimeric proteins suggest that the catalytic and/or C-terminal domains give CesA1 and CesA3 access to their specific sites in the cellulose synthase of primary walls

Wang, Jian; Howles, Paul; Cork, Ann; Birch, Rosemary; Williamson, Richard

Description

CesA1 and CesA3 are thought to occupy noninterchangeable sites in the cellulose synthase making primary wall cellulose in Arabidopsis (Arabidopsis thaliana L. Heynh). With domain swaps and deletions, we show that sites C terminal to transmembrane domain 2 give CesAs access to their individual sites and, from dominance and recessive behavior, deduce that certain CesA alleles exclude others from accessing each site. Constructs that swapped or deleted N-terminal domains were stably transformed...[Show more]

dc.contributor.authorWang, Jian
dc.contributor.authorHowles, Paul
dc.contributor.authorCork, Ann
dc.contributor.authorBirch, Rosemary
dc.contributor.authorWilliamson, Richard
dc.date.accessioned2015-12-07T22:53:56Z
dc.identifier.issn0032-0889
dc.identifier.urihttp://hdl.handle.net/1885/27944
dc.description.abstractCesA1 and CesA3 are thought to occupy noninterchangeable sites in the cellulose synthase making primary wall cellulose in Arabidopsis (Arabidopsis thaliana L. Heynh). With domain swaps and deletions, we show that sites C terminal to transmembrane domain 2 give CesAs access to their individual sites and, from dominance and recessive behavior, deduce that certain CesA alleles exclude others from accessing each site. Constructs that swapped or deleted N-terminal domains were stably transformed into the wild type and into the temperature-sensitive mutants rsw1 (Ala-549Val in CesA1) and rsw5 (Pro-1056Ser in CesA3). Dominant-positive behavior was assayed as root elongation at the restrictive temperature and dominant-negative effects were observed at the permissive temperature. A protein with the catalytic and C-terminal domains of CesA1 and the N-terminal domain of CesA3 promoted growth only in rsw1 consistent with it accessing the CesA1 site even though it contained the CesA3 N-terminal domain. A protein having the CesA3 catalytic and C-terminal domains linked to the CesA1 N-terminal domain dramatically affected growth, but only in the CesA3 mutant. This is consistent with the operation of the same access rule taking this chimeric protein to the CesA3 site. In this case, however, the transgene behaved as a genotype-specific dominant negative, causing a 60% death rate in rsw5, but giving no visible phenotype in wild type or rsw1. We therefore hypothesize that possession of CesA3WT protects Columbia and rsw1 from the lethal effects of this chimeric protein, whereas the mutant protein (CesA3rsw5) does not.
dc.publisherAmerican Society of Plant Biologists
dc.sourcePlant Physiology
dc.subjectKeywords: Cellulose synthase; Chimeric proteins; Catalysis; Cell membranes; Enzymes; Plant cell culture; Proteins; Arabidopsis protein; cellulose; cellulose synthase; CESA1 protein, Arabidopsis; CesA3 protein, Arabidopsis; chimeric protein; glucosyltransferase; ami
dc.titleChimeric proteins suggest that the catalytic and/or C-terminal domains give CesA1 and CesA3 access to their specific sites in the cellulose synthase of primary walls
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume142
dc.date.issued2006
local.identifier.absfor060103 - Cell Development, Proliferation and Death
local.identifier.ariespublicationu9204316xPUB54
local.type.statusPublished Version
local.contributor.affiliationWang, Jian, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHowles, Paul, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationCork, Ann, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBirch, Rosemary, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWilliamson, Richard, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage685
local.bibliographicCitation.lastpage695
local.identifier.doi10.1104/pp.106.084004
dc.date.updated2015-12-07T12:45:06Z
local.identifier.scopusID2-s2.0-33750049646
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Wang_Chimeric_proteins_suggest_that_2006.pdf916.79 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator