Intensity isotherms and distributions on oligonucleotide microarrays
We describe a physico-chemical model relating measured fluorescence intensities on oligonucleotide microarrays to the underlying specific target concentration in the hybridised solution via a hyperbolic isotherm response function. The model includes various chemical reactions occurring at the microarray surface and in bulk solution during hybridisation, including specific and non-specific hybridisation, and also the effects of probe-target dissociation during the post hybridisation washing...[Show more]
|Collections||ANU Research Publications|
|Source:||2009 IEEE Congress on Evolutionary Computation Proceedings|
|01_Burden_Intensity_isotherms_and_2009.pdf||1.11 MB||Adobe PDF||Request a copy|
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