Loscha, Karin; Oakley, Aaron; Bancia, Bogdan; Schaeffer, Patrick; Prosselkov, Pavel; Otting, Gottfried; Wilce, Matthew; Dixon, Nicholas
In Escherichia coli, the DnaG primase is the RNA polymerase that synthesizes RNA primers at replication forks. It is composed of three domains, a small N-terminal zinc-binding domain, a larger central domain responsible for RNA synthesis, and a C-terminal domain comprising residues 434-581 [DnaG(434-581)] that interact with the hexameric DnaB helicase. Presumably because of this interaction, it had not been possible previously to express the C-terminal domain in a stably transformed E. coli...[Show more]
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