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Directed evolution of trimethoprim resistance in Escherichia coli

Watson, Morgan; Liu, Jian-Wei; Ollis, David


Directed evolution is a useful tool in the study of enzymes. It is used in this study to investigate the means by which resistance to the antibiotic trimethoprim develops in dihyrofolate reductase from Escherichia coli. Mutants with clinical levels of resistance were obtained after only three generations. After four generations of directed evolution, several mutants were characterized, along with some point mutants made to investigate amino acid changes of interest. Several mutations were found...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: The FEBS Journal
DOI: 10.1111/j.1742-4658.2007.05801.x


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