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A multitiered sequence capture strategy spanning broad evolutionary scales: Application for phylogenetic and phylogeographic studies of orchids

Peakall, Rod; Wong, Darren; Phillips, Ryan; RUIBAL, MONICA; Eyles, Rodney; Rodriguez Delgado, Claudia; Linde, Celeste

Description

With over 25,000 species, the drivers of diversity in the Orchidaceae remain to be fully understood. Here, we outline a multitiered sequence capture strategy aimed at capturing hundreds of loci to enable phylogenetic resolution from subtribe to subspecific levels in orchids of the tribe Diurideae. For the probe design, we mined subsets of 18 transcriptomes, to give five target sequence sets aimed at the tribe (Sets 1 & 2), subtribe (Set 3), and within subtribe levels (Sets 4 & 5). Analysis...[Show more]

CollectionsANU Research Publications
Date published: 2021
Type: Journal article
URI: http://hdl.handle.net/1885/272361
Source: Molecular Ecology Resources
DOI: 10.1111/1755-0998.13327

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