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Identification of the endangered Australian orchid Microtis angusii using an allele-specific PCR assay

Flanagan, Nicola; Peakall, Rodney; Clements, M A; Otero, J Tupac


The endangered orchid, Microtis angusii, was recently described from a single population consisting of approximately 100 plants. This species is morphologically very similar to close relatives, and taxonomic difficulties have hindered attempts to identify further populations for protection. Here we present a rapid, economical, PCR-based assay for the effective identification of this species based on rDNA sequence variation. Using two single nucleotide substitutions in the internal transcribed...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: Conservation Genetics
DOI: 10.1007/s10592-006-9198-6


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