Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds
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Barrero , Jose; Millar, Anthony; Griffiths , Jayne; Czechowski, Tomasz; Scheible, Wolf R; Udvardi, Michael; Reid, John B; Ross, John J; Jacobsen , John V; Gubler, Frank; Jacobsen, J; Jacobsen, John
Description
Seed dormancy is a very important trait that maximizes the survival of seed in nature, the control of which can have important repercussions on the yield of many crop species. We have used gene expression profiling to identify genes that are involved in dormancy regulation in Arabidopsis thaliana. RNA was isolated from imbibed dormant (D) and after-ripened (AR) ecotype C24 seeds, and then screened by quantitative RT-PCR (qRT-PCR) for differentially expressed transcription factors (TFs) and...[Show more]
dc.contributor.author | Barrero , Jose | |
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dc.contributor.author | Millar, Anthony | |
dc.contributor.author | Griffiths , Jayne | |
dc.contributor.author | Czechowski, Tomasz | |
dc.contributor.author | Scheible, Wolf R | |
dc.contributor.author | Udvardi, Michael | |
dc.contributor.author | Reid, John B | |
dc.contributor.author | Ross, John J | |
dc.contributor.author | Jacobsen , John V | |
dc.contributor.author | Gubler, Frank | |
dc.contributor.author | Jacobsen, J | |
dc.contributor.author | Jacobsen, John | |
dc.date.accessioned | 2015-12-07T22:49:42Z | |
dc.identifier.issn | 0960-7412 | |
dc.identifier.uri | http://hdl.handle.net/1885/26884 | |
dc.description.abstract | Seed dormancy is a very important trait that maximizes the survival of seed in nature, the control of which can have important repercussions on the yield of many crop species. We have used gene expression profiling to identify genes that are involved in dormancy regulation in Arabidopsis thaliana. RNA was isolated from imbibed dormant (D) and after-ripened (AR) ecotype C24 seeds, and then screened by quantitative RT-PCR (qRT-PCR) for differentially expressed transcription factors (TFs) and other regulatory genes. Out of 2207 genes screened, we have identified 39 that were differentially expressed during the first few hours of imbibition. After analyzing T-DNA insertion mutants for 22 of these genes, two displayed altered dormancy compared with the wild type. These mutants are affected in genes that encode a RING finger and an HDZip protein. The first, named DESPIERTO, is involved in ABA sensitivity during seed development, regulates the expression of ABI3, and produces a complete loss of dormancy when mutated. The second, the HDZip (ATHB20), is expressed during seed germination in the micropylar endosperm and in the root cap, and increases ABA sensitivity and seed dormancy when mutated. | |
dc.publisher | Blackwell Publishing Ltd | |
dc.source | The Plant Journal | |
dc.subject | Keywords: ABI3 protein, Arabidopsis; abscisic acid; Arabidopsis protein; bacterial DNA; HD Zip protein, Arabidopsis; HD-Zip protein, Arabidopsis; homeodomain protein; plant RNA; T DNA; T-DNA; transcription factor; Arabidopsis; article; gene expression profiling; ge ABA; ATHB20; Germination; Seed dormancy; Transcription factor profiling | |
dc.title | Gene expression profiling identifies two regulatory genes controlling dormancy and ABA sensitivity in Arabidopsis seeds | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 61 | |
dc.date.issued | 2010 | |
local.identifier.absfor | 060705 - Plant Physiology | |
local.identifier.absfor | 060702 - Plant Cell and Molecular Biology | |
local.identifier.ariespublication | u4956746xPUB47 | |
local.type.status | Published Version | |
local.contributor.affiliation | Barrero , Jose , CSIRO Plant Industry | |
local.contributor.affiliation | Millar, Anthony, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Griffiths , Jayne , CSIRO Plant Industry | |
local.contributor.affiliation | Czechowski, Tomasz, Max Planck Institue of Molecular Plant Physiology | |
local.contributor.affiliation | Scheible, Wolf R, Max Planck Institute of Molecular Plant Physiology | |
local.contributor.affiliation | Udvardi, Michael, Max Planck Institute of Molecular Plant Physiology | |
local.contributor.affiliation | Reid, John B , University of Tasmania | |
local.contributor.affiliation | Ross, John J, University of Tasmania | |
local.contributor.affiliation | Jacobsen , John V , CSIRO Plant Industry | |
local.contributor.affiliation | Gubler, Frank , CSIRO | |
local.contributor.affiliation | Jacobsen, J, CSIRO Plant Industry | |
local.contributor.affiliation | Jacobsen, John, CSIRO Plant Industry | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 4 | |
local.bibliographicCitation.startpage | 611 | |
local.bibliographicCitation.lastpage | 622 | |
local.identifier.doi | 10.1111/j.1365-313X.2009.04088.x | |
local.identifier.absseo | 829999 - Plant Production and Plant Primary Products not elsewhere classified | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2024-03-03T07:18:35Z | |
local.identifier.scopusID | 2-s2.0-76449108420 | |
local.identifier.thomsonID | 000274336900007 | |
Collections | ANU Research Publications |
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