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High-molecular weight DNA extraction, clean-up and size selection for long-read sequencing

Jones, Ashley; Torkel, Cynthia; Stanley, David; Nasim, Jamila; Borevitz, Justin; Schwessinger, Benjamin


Rapid advancements in long-read sequencing technologies have transformed read lengths from bps to Mbps, which has enabled chromosome-scale genome assemblies. However, read lengths are now becoming limited by the extraction of pure high-molecular weight DNA suitable for long-read sequencing, which is particularly challenging in plants and fungi. To overcome this, we present a protocol collection; high-molecular weight DNA extraction, clean-up and size selection for long-read sequencing. We...[Show more]

CollectionsANU Research Publications
Date published: 2021-07-15
Type: Journal article
Source: PLOS ONE
DOI: 10.1371/journal.pone.0253830
Access Rights: Open Access


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