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Patterns of Gene Expression in a Scleractinian Coral Undergoing Natural Bleaching

Seneca, Francois O; Foret, Sylvain; Ball, Eldon; Smith-Keune, Carolyn; Miller, David J.; van Oppen, Madeleine

Description

Coral bleaching is a major threat to coral reefs worldwide and is predicted to intensify with increasing global temperature. This study represents the first investigation of gene expression in an Indo-Pacific coral species undergoing natural bleaching which involved the loss of algal symbionts. Quantitative real-time polymerase chain reaction experiments were conducted to select and evaluate coral internal control genes (ICGs), and to investigate selected coral genes of interest (GOIs) for...[Show more]

dc.contributor.authorSeneca, Francois O
dc.contributor.authorForet, Sylvain
dc.contributor.authorBall, Eldon
dc.contributor.authorSmith-Keune, Carolyn
dc.contributor.authorMiller, David J.
dc.contributor.authorvan Oppen, Madeleine
dc.date.accessioned2015-12-07T22:48:00Z
dc.identifier.issn1436-2228
dc.identifier.urihttp://hdl.handle.net/1885/26298
dc.description.abstractCoral bleaching is a major threat to coral reefs worldwide and is predicted to intensify with increasing global temperature. This study represents the first investigation of gene expression in an Indo-Pacific coral species undergoing natural bleaching which involved the loss of algal symbionts. Quantitative real-time polymerase chain reaction experiments were conducted to select and evaluate coral internal control genes (ICGs), and to investigate selected coral genes of interest (GOIs) for changes in gene expression in nine colonies of the scleractinian coral Acropora millepora undergoing bleaching at Magnetic Island, Great Barrier Reef, Australia. Among the six ICGs tested, glyceraldehyde 3-phosphate dehydrogenase and the ribosomal protein genes S7 and L9 exhibited the most constant expression levels between samples from healthy-looking colonies and samples from the same colonies when severely bleached a year later. These ICGs were therefore utilised for normalisation of expression data for seven selected GOIs. Of the seven GOIs, homologues of catalase, C-type lectin and chromoprotein genes were significantly up-regulated as a result of bleaching by factors of 1.81, 1.46 and 1.61 (linear mixed models analysis of variance, P < 0.05), respectively. We present these genes as potential coral bleaching response genes. In contrast, three genes, including one putative ICG, showed highly variable levels of expression between coral colonies. Potential variation in microhabitat, gene function unrelated to the stress response and individualised stress responses may influence such differences between colonies and need to be better understood when designing and interpreting future studies of gene expression in natural coral populations.
dc.publisherSpringer
dc.sourceMarine Biotechnology
dc.subjectKeywords: Coral bleaching; Inter-colony variability; Internal controls; Molecular stress; Quantitative PCR; Bleaching; Cleaning; Finance; Polymerase chain reaction; Reefs; Regression analysis; Gene expression; proteome; bioassay; coral; coral bleaching; coral reef; Coral bleaching; Gene expression; Inter-colony variability; Internal control gene; Molecular stress response; Quantitative PCR
dc.titlePatterns of Gene Expression in a Scleractinian Coral Undergoing Natural Bleaching
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolumeOnline
dc.date.issued2009
local.identifier.absfor060306 - Evolutionary Impacts of Climate Change
local.identifier.absfor060405 - Gene Expression (incl. Microarray and other genome-wide approaches)
local.identifier.absfor060806 - Animal Physiological Ecology
local.identifier.ariespublicationu8611701xPUB43
local.type.statusPublished Version
local.contributor.affiliationSeneca, Francois O, James Cook University
local.contributor.affiliationForet, Sylvain, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationBall, Eldon, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSmith-Keune, Carolyn, Australian Institute of Marine Science
local.contributor.affiliationMiller, David J., James Cook University
local.contributor.affiliationvan Oppen, Madeleine, Australian Institute of Marine Science
local.description.embargo2037-12-31
local.bibliographicCitation.startpage11
local.identifier.doi10.1007/s10126-009-9247-5
dc.date.updated2016-02-24T11:42:22Z
local.identifier.scopusID2-s2.0-77957754121
local.identifier.thomsonID000282592900012
CollectionsANU Research Publications

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