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Molecular analysis of Shigella boydii O1 O-antigen gene cluster and its PCR typing

Tao, Jiang; Wang, Lei; Liu, Dan; Bastin, David A; Genge, Yunqi; Feng, Lu

Description

Shigella is an important human pathogen and is closely related to Escherichia coli. O-antigen is the most variable part of the lipopolysaccharide on the cell surface of Gram-negative bacteria and plays an important role in pathogenicity. The O-antigen gene cluster of S. boydii O1 was sequenced. The putative genes encoding enzymes for rhamnose synthesis, transferases, O-unit flippase, and O-unit polymerase were identified on the basis of homology. The O-antigen gene clusters of S. boydii O1 and...[Show more]

dc.contributor.authorTao, Jiang
dc.contributor.authorWang, Lei
dc.contributor.authorLiu, Dan
dc.contributor.authorBastin, David A
dc.contributor.authorGenge, Yunqi
dc.contributor.authorFeng, Lu
dc.date.accessioned2015-12-07T22:47:54Z
dc.identifier.issn1480-3275
dc.identifier.urihttp://hdl.handle.net/1885/26255
dc.description.abstractShigella is an important human pathogen and is closely related to Escherichia coli. O-antigen is the most variable part of the lipopolysaccharide on the cell surface of Gram-negative bacteria and plays an important role in pathogenicity. The O-antigen gene cluster of S. boydii O1 was sequenced. The putative genes encoding enzymes for rhamnose synthesis, transferases, O-unit flippase, and O-unit polymerase were identified on the basis of homology. The O-antigen gene clusters of S. boydii O1 and E. coli O149, which share the same O-antigen form, were found to have the same genes and organization by adjacent gene PCR assay. Two genes specific for S. boydii O1 and E. coli O149 were identified by PCR screening against E. coli- and Shigella-type strains of the 186 known O-antigen forms and 39 E. coli clinical isolates. A PCR sensitivity of 103 to 104 CFU/mL overnight culture of S. boydii O1 and E. coli O149 was obtained. S. boydii O1 and E. coli O149 were differentiated by PCR using lacZ- and cadA-based primers.
dc.publisherNRC Research Press
dc.sourceCanadian Journal of Microbiology
dc.subjectKeywords: Bacteria; Bioassay; Enzymes; Escherichia coli; Genes; Molecular biology; Polysaccharides; Synthesis (chemical); E. Coli 0149; Molecular typing; O-antigen gene cluster; S. Boydii 01; Antigens; beta galactosidase; DNA directed DNA polymerase alpha; enzyme; E. Coli O149; Molecular typing; O-antigen gene cluster; S. Boydii O1
dc.titleMolecular analysis of Shigella boydii O1 O-antigen gene cluster and its PCR typing
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume51
dc.date.issued2005
local.identifier.absfor060500 - MICROBIOLOGY
local.identifier.ariespublicationu4692404xPUB43
local.type.statusPublished Version
local.contributor.affiliationTao, Jiang, Nankai University
local.contributor.affiliationWang, Lei, Nankai University
local.contributor.affiliationLiu, Dan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBastin, David A, Tianjin Biochip Company
local.contributor.affiliationGenge, Yunqi, Nankai University
local.contributor.affiliationFeng, Lu, Nankai University
local.description.embargo2037-12-31
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage387
local.bibliographicCitation.lastpage393
local.identifier.doi10.1139/w05-015
dc.date.updated2015-12-07T11:55:18Z
local.identifier.scopusID2-s2.0-27744514460
CollectionsANU Research Publications

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