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The human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in somatic cells

Keam, Simon P; Young, Paul E; McCorkindale, Alexandra L; Dang, Thurston H Y; Clancy, Jennifer; Humphreys, David T; Preiss, Thomas; Hutvagner, Gyorgy; Martin, David I K; Cropley, Jennifer E; Suter, Catherine M

Description

The Piwi-piRNA pathway is active in animal germ cells where its functions are required for germ cell maintenance and gamete differentiation. Piwi proteins and piRNAs have been detected outside germline tissue in multiple phyla, but activity of the pathway in mammalian somatic cells has been little explored. In particular, Piwi expression has been observed in cancer cells, but nothing is known about the piRNA partners or the function of the system in these cells. We have surveyed the expression...[Show more]

dc.contributor.authorKeam, Simon P
dc.contributor.authorYoung, Paul E
dc.contributor.authorMcCorkindale, Alexandra L
dc.contributor.authorDang, Thurston H Y
dc.contributor.authorClancy, Jennifer
dc.contributor.authorHumphreys, David T
dc.contributor.authorPreiss, Thomas
dc.contributor.authorHutvagner, Gyorgy
dc.contributor.authorMartin, David I K
dc.contributor.authorCropley, Jennifer E
dc.contributor.authorSuter, Catherine M
dc.date.accessioned2015-12-07T22:47:45Z
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/1885/26186
dc.description.abstractThe Piwi-piRNA pathway is active in animal germ cells where its functions are required for germ cell maintenance and gamete differentiation. Piwi proteins and piRNAs have been detected outside germline tissue in multiple phyla, but activity of the pathway in mammalian somatic cells has been little explored. In particular, Piwi expression has been observed in cancer cells, but nothing is known about the piRNA partners or the function of the system in these cells. We have surveyed the expression of the three human Piwi genes, Hiwi, Hili and Hiwi2, in multiple normal tissues and cancer cell lines. We find that Hiwi2 is ubiquitously expressed; in cancer cells the protein is largely restricted to the cytoplasm and is associated with translating ribosomes. Immunoprecipitation of Hiwi2 from MDAMB231 cancer cells enriches for piRNAs that are predominantly derived from processed tRNAs and expressed genes, species which can also be found in adult human testis. Our studies indicate that a Piwi-piRNA pathway is present in human somatic cells, with an uncharacterised function linked to translation. Taking this evidence together with evidence from primitive organisms, we propose that this somatic function of the pathway predates the germline functions of the pathway in modern animals.
dc.publisherOxford University Press
dc.rightsAuthor/s retain copyright
dc.sourceNucleic Acids Research
dc.titleThe human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in somatic cells
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume42
dc.date.issued2014
local.identifier.absfor060104 - Cell Metabolism
local.identifier.ariespublicationu3526593xPUB43
local.type.statusPublished Version
local.contributor.affiliationKeam, Simon P, Victor Chang Cardiac Research Institute
local.contributor.affiliationYoung, Paul E, Victor Change Cardiac Research Institute
local.contributor.affiliationMcCorkindale, Alexandra L, Victor Chang Cardiac Research Institute
local.contributor.affiliationDang, Thurston H Y, Victor Chang Cardiac Research Institute
local.contributor.affiliationClancy, Jennifer, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHumphreys, David T, Victor Chang Chardiac Research Institute
local.contributor.affiliationPreiss, Thomas, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHutvagner, Gyorgy, University of Technology
local.contributor.affiliationMartin, David I K, Children's Hospital Oakland Institute
local.contributor.affiliationCropley, Jennifer E, Victor Chang Cardiac Research Institute
local.contributor.affiliationSuter, Catherine M, Victor Chang Cardiac Research Institute
local.bibliographicCitation.issue14
local.bibliographicCitation.startpage8984
local.bibliographicCitation.lastpage8995
local.identifier.doi10.1093/nar/gku620
dc.date.updated2015-12-07T11:53:42Z
local.identifier.scopusID2-s2.0-84906263034
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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