Man, Bradley; Su, Xun-Cheng; Liang, Haobo; Simonsen, Shane; Huber, Thomas; Messerle, Barbara Ann; Otting, Gottfried
Paramagnetic effects from lanthanide ions present powerful tools for protein studies by nuclear magnetic resonance (NMR) spectroscopy provid-ed that the lanthanide can be site-spe-cifically and rigidly attached to the pro-tein. A new, particularly small and rigid lanthanide-binding tag, 3-mercap-to-2, 6-pyridinedicarboxylic acid (3MDPA), was synthesized and at-tached to two different proteins via a disulfide bond. The complexes of the N-terminal domain of the E. coli argi-nine repressor (ArgN)...[Show more]
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