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HiRIEF LC-MS enables deep proteome coverage and unbiased proteogenomics

Branca, Rui M M; Orre, Lukas M; Johansson, Henrik J; Granholm, Viktor; Huss, Mikael; Perez-Bercoff, Asa; Forshed, Jenny; Käll, Lukas; Lehtiö, Janne

Description

We present a liquid chromatography-mass spectrometry (LC-MS)-based method permitting unbiased (gene prediction-independent) genome-wide discovery of protein-coding loci in higher eukaryotes. Using high-resolution isoelectric focusing (HiRIEF) at the peptide level in the 3.7-5.0 pH range and accurate peptide isoelectric point (pI) prediction, we probed the six-reading-frame translation of the human and mouse genomes and identified 98 and 52 previously undiscovered protein-coding loci,...[Show more]

dc.contributor.authorBranca, Rui M M
dc.contributor.authorOrre, Lukas M
dc.contributor.authorJohansson, Henrik J
dc.contributor.authorGranholm, Viktor
dc.contributor.authorHuss, Mikael
dc.contributor.authorPerez-Bercoff, Asa
dc.contributor.authorForshed, Jenny
dc.contributor.authorKäll, Lukas
dc.contributor.authorLehtiö, Janne
dc.date.accessioned2015-12-07T22:46:15Z
dc.identifier.issn1548-7091
dc.identifier.urihttp://hdl.handle.net/1885/25698
dc.description.abstractWe present a liquid chromatography-mass spectrometry (LC-MS)-based method permitting unbiased (gene prediction-independent) genome-wide discovery of protein-coding loci in higher eukaryotes. Using high-resolution isoelectric focusing (HiRIEF) at the peptide level in the 3.7-5.0 pH range and accurate peptide isoelectric point (pI) prediction, we probed the six-reading-frame translation of the human and mouse genomes and identified 98 and 52 previously undiscovered protein-coding loci, respectively. The method also enabled deep proteome coverage, identifying 13,078 human and 10,637 mouse proteins.
dc.publisherNature Publishing Group
dc.sourceNature Methods: techniques for life scientists and chemists
dc.titleHiRIEF LC-MS enables deep proteome coverage and unbiased proteogenomics
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume11
dc.date.issued2014
local.identifier.absfor060109 - Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
local.identifier.ariespublicationu3526593xPUB40
local.type.statusPublished Version
local.contributor.affiliationBranca, Rui M M, Karolinska Institutet
local.contributor.affiliationOrre, Lukas M, Karolinska Institutet
local.contributor.affiliationJohansson, Henrik J, Karolinska Institutet
local.contributor.affiliationGranholm, Viktor, Stockholm University
local.contributor.affiliationHuss, Mikael, Stockholm University
local.contributor.affiliationPerez-Bercoff, Asa, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationForshed, Jenny, Karolinska Institutet
local.contributor.affiliationKäll, Lukas, KTH Royal Institute of Technology
local.contributor.affiliationLehtiö, Janne, Karolinska Institutet
local.description.embargo2037-12-31
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage59
local.bibliographicCitation.lastpage62
local.identifier.doi10.1038/nmeth.2732
dc.date.updated2015-12-07T11:39:25Z
local.identifier.scopusID2-s2.0-84894619287
CollectionsANU Research Publications

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