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Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

Quah, Ben; Warren, Hilary; Parish, Christopher


This protocol outlines the carboxyfluorescein diacetate succinimidyl ester (CFSE) method for following the proliferation of human lymphocytes in vitro and mouse lymphocytes both in vitro and in vivo. The method relies on the ability of CFSE to covalently

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: Nature Protocols
DOI: 10.1038/nprot.2007.296


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