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Scanning transmission ion micro-tomography (STIM-T) of biological specimens

Sakellariou, Arthur; Schwertner, Michael; Reinert, Tilo; Butz, Tilman

Description

Computed tomography (CT) was applied to sets of Scanning Transmission Ion Microscopy (STIM) projections recorded at the LIPSION ion beam laboratory (Leipzig) in order to visualize the 3D-mass distribution in several specimens. Examples for a test structure (copper grid) and for biological specimens (cartilage cells, cygospore) are shown. Scanning Transmission Micro-Tomography (STIM-T) at a resolution of 260 nm was demonstrated for the first time. Sub-micron features of the Cu-grid specimen were...[Show more]

dc.contributor.authorSakellariou, Arthur
dc.contributor.authorSchwertner, Michael
dc.contributor.authorReinert, Tilo
dc.contributor.authorButz, Tilman
dc.date.accessioned2015-12-07T22:46:01Z
dc.identifier.issn0304-3991
dc.identifier.urihttp://hdl.handle.net/1885/25599
dc.description.abstractComputed tomography (CT) was applied to sets of Scanning Transmission Ion Microscopy (STIM) projections recorded at the LIPSION ion beam laboratory (Leipzig) in order to visualize the 3D-mass distribution in several specimens. Examples for a test structure (copper grid) and for biological specimens (cartilage cells, cygospore) are shown. Scanning Transmission Micro-Tomography (STIM-T) at a resolution of 260 nm was demonstrated for the first time. Sub-micron features of the Cu-grid specimen were verified by scanning electron microscopy. The ion energy loss measured during a STIM-T experiment is related to the mass density of the specimen. Typically, biological specimens can be analysed without staining. Only shock freezing and freeze-drying is required to preserve the ultra-structure of the specimen. The radiation damage to the specimen during the experiment can be neglected. This is an advantage compared to other techniques like X-ray micro-tomography. At present, the spatial resolution is limited by beam position fluctuations and specimen vibrations.
dc.publisherElsevier
dc.sourceUltramicroscopy
dc.subjectKeywords: Energy dissipation; Ion beams; Ion microscopes; Radiation damage; Scanning electron microscopy; Biological specimen; Energy loss projection; Focussed ion beams; Scanning Transmission Ion Microscopy (STIM); Sub-micron resolution; Computerized tomography; a Biological specimen; Energy loss projection; Focussed ion beam; STIM-T; Sub-micron resolution; Tomography
dc.titleScanning transmission ion micro-tomography (STIM-T) of biological specimens
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume106
dc.date.issued2006
local.identifier.absfor069999 - Biological Sciences not elsewhere classified
local.identifier.ariespublicationu4441299xPUB39
local.type.statusPublished Version
local.contributor.affiliationSakellariou, Arthur, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationSchwertner, Michael, University of Leipzig
local.contributor.affiliationReinert, Tilo, University of Leipzig
local.contributor.affiliationButz, Tilman, University of Leipzig
local.description.embargo2037-12-31
local.bibliographicCitation.issue7
local.bibliographicCitation.startpage574
local.bibliographicCitation.lastpage581
local.identifier.doi10.1016/j.ultramic.2006.02.003
dc.date.updated2015-12-07T11:36:34Z
local.identifier.scopusID2-s2.0-33746700923
CollectionsANU Research Publications

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