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NMR detection of protein 15 N spins near paramagnetic lanthanide ions

John, Michael; Park, Ah Young; Dixon, Nicholas; Otting, Gottfried


Pronounced paramagnetic relaxation enhancement (PRE) due to paramagnetic metal ions prevents the observation of NMR signals from 1H spins near the metal. While 15N spins are less prone to PRE, the intrinsic sensitivity of 15N NMR spectroscopy is low. This Communication presents a 1H detected out-and-back Nz-exchange experiment which allows the measurement of pseudocontact shifts of 15N spins located as close as 6 Å from a Dy3+ ion in a 30 kDa protein complex. The experiment relies on the...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: Journal of the American Chemical Society
DOI: 10.1021/ja066995o


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