John, Michael; Park, Ah Young; Dixon, Nicholas; Otting, Gottfried
Pronounced paramagnetic relaxation enhancement (PRE) due to paramagnetic metal ions prevents the observation of NMR signals from 1H spins near the metal. While 15N spins are less prone to PRE, the intrinsic sensitivity of 15N NMR spectroscopy is low. This Communication presents a 1H detected out-and-back Nz-exchange experiment which allows the measurement of pseudocontact shifts of 15N spins located as close as 6 Å from a Dy3+ ion in a 30 kDa protein complex. The experiment relies on the...[Show more]
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