Site-specific incorporation of unnatural amino acids into proteins by cell-free protein synthesis
Cell-free protein synthesis (CFPS) offers a fast and inexpensive means to incorporate unnatural amino acids (UAAs) site specifically into proteins. This enables engineering of proteins and allows production of protein-based probes for analysis of their interactions with other molecules. Using dialysis Escherichia coli CFPS system in combination with aminoacyl-tRNA synthetase and suppressor tRNA evolved from Methanocaldococcus jannaschii high expression yield of proteins with site specifically...[Show more]
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|Source:||Methods in Molecular Biology|
|01_Ozawa_Site-specific_incorporation_of_2014.pdf||1.16 MB||Adobe PDF||Request a copy|
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