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Site-specific incorporation of unnatural amino acids into proteins by cell-free protein synthesis

Ozawa, Kiyoshi; Loh, Choy


Cell-free protein synthesis (CFPS) offers a fast and inexpensive means to incorporate unnatural amino acids (UAAs) site specifically into proteins. This enables engineering of proteins and allows production of protein-based probes for analysis of their interactions with other molecules. Using dialysis Escherichia coli CFPS system in combination with aminoacyl-tRNA synthetase and suppressor tRNA evolved from Methanocaldococcus jannaschii high expression yield of proteins with site specifically...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
Source: Methods in Molecular Biology
DOI: 10.1007/978-1-62703-782-2_12


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