Serum- and glucocorticoid-dependent kinase, cell volume, and the regulation of epithelial transport
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Fillon, S; Warntges, Simone; Matskevitch, J; Moschen, I; Setiawan, Iwan; Gamper, N; Feng, Y-X; Stegen, C; Friedrich, Bjorn; Waldegger, S; Broer, Stefan
Description
the regulation of cell volume. Given the limited selectivity of most inhibitors, however, the specific molecules involved have remained largely elusive. The search for cell volume regulated genes in liver HepG2 cells led to the discovery of the human serum- and glucocorticoid-dependent serine/threonine kinase hsgk1. Transcription and expression of hsgk1 is markedly and rapidly upregulated by osmotic and isotonic cell shrinkage. The effect of osmotic cell shrinkage on hsgk1 is mediated by p38...[Show more]
dc.contributor.author | Fillon, S | |
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dc.contributor.author | Warntges, Simone | |
dc.contributor.author | Matskevitch, J | |
dc.contributor.author | Moschen, I | |
dc.contributor.author | Setiawan, Iwan | |
dc.contributor.author | Gamper, N | |
dc.contributor.author | Feng, Y-X | |
dc.contributor.author | Stegen, C | |
dc.contributor.author | Friedrich, Bjorn | |
dc.contributor.author | Waldegger, S | |
dc.contributor.author | Broer, Stefan | |
dc.date.accessioned | 2015-12-07T22:44:13Z | |
dc.identifier.issn | 1095-6433 | |
dc.identifier.uri | http://hdl.handle.net/1885/25098 | |
dc.description.abstract | the regulation of cell volume. Given the limited selectivity of most inhibitors, however, the specific molecules involved have remained largely elusive. The search for cell volume regulated genes in liver HepG2 cells led to the discovery of the human serum- and glucocorticoid-dependent serine/threonine kinase hsgk1. Transcription and expression of hsgk1 is markedly and rapidly upregulated by osmotic and isotonic cell shrinkage. The effect of osmotic cell shrinkage on hsgk1 is mediated by p38 kinase. Further stimuli of hsgk1 transcription include glucocorticoids, aldosterone, TGF-β1, serum, increase of intracellular Ca2+ and phorbolesters, whereas cAMP downregulates hsgk1 transcription. The hsgk1 protein is expressed in several epithelial tissues including human pancreas, intestine, kidney, and shark rectal gland. Co-expression of hsgk1 with the renal epithelial Na+-channel ENaC or the Na+/K+/2Cl--cotransporter NKCC2 (BSC1) in Xenopus oocytes, accelerates insertion of the transport proteins into the cell membrane and thus, stimulates channel or transport activity. Thus, hsgk1 participates in the regulation of transport by steroids and secretagogues increasing intracellular Ca2+-activity. The stimulation of hsgk1 transcription by TGF-β1 may further bear pathophysiological relevance. | |
dc.publisher | Elsevier | |
dc.source | Comparative Biochemistry and Physiology A: Molecular and Integrative Physiology | |
dc.subject | Keywords: aldosterone; calcium; carrier protein; chloride; cyclic AMP; glucocorticoid; isotonic solution; phorbol ester derivative; phosphotransferase; phosphotransferase inhibitor; potassium; protein kinase p38; protein serine threonine kinase; sodium; sodium chan Cell volume regulation; Epithelial Na+ channel; Intestine; Intracellular Ca2+ activity; Kidney; Na+/K+/2Cl--cotransporter; Pancreas; Sgk | |
dc.title | Serum- and glucocorticoid-dependent kinase, cell volume, and the regulation of epithelial transport | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 130 | |
dc.date.issued | 2001 | |
local.identifier.absfor | 060110 - Receptors and Membrane Biology | |
local.identifier.ariespublication | u4325460xPUB36 | |
local.type.status | Published Version | |
local.contributor.affiliation | Fillon, S, University of Tubingen | |
local.contributor.affiliation | Warntges, Simone, University of Tubingen | |
local.contributor.affiliation | Matskevitch, J, University of Tubingen | |
local.contributor.affiliation | Moschen, I, University of Tubingen | |
local.contributor.affiliation | Setiawan, Iwan, University of Tubingen | |
local.contributor.affiliation | Gamper, N, University of Tubingen | |
local.contributor.affiliation | Feng, Y-X, University of Queensland | |
local.contributor.affiliation | Stegen, C, University of Tubingen | |
local.contributor.affiliation | Friedrich, Bjorn, University of Tubingen | |
local.contributor.affiliation | Waldegger, S, University of Tubingen | |
local.contributor.affiliation | Broer, Stefan, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 367 | |
local.bibliographicCitation.lastpage | 376 | |
local.identifier.doi | 10.1016/S1095-6433(01)00422-6 | |
dc.date.updated | 2015-12-07T11:22:09Z | |
local.identifier.scopusID | 2-s2.0-0034792780 | |
Collections | ANU Research Publications |
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