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Target RNA Secondary Structure Is a Major Determinant of miR159 Efficacy

ZHENG, ZIHUI; Reichel, Marlene; Deveson, Ira; Wong, Gigi; Millar, Anthony; Li, Junyan

Description

In plants, microRNA (miRNA)-target complementarity has long been considered the predominant factor determining the silencing outcome of the miRNA-target interaction, although the efficacy of such interactions have rarely been appraised in plants. Here, we perform in planta silencing efficacy assays on seven Arabidopsis MYB genes, all of which contain conserved miR159-binding sites of analogous complementarity. These genes were found to be differentially silenced by miR159; MYB81, MYB97, MYB101,...[Show more]

CollectionsANU Research Publications
Date published: 2017-05-17
Type: Journal article
URI: http://hdl.handle.net/1885/247772
Source: Plant Physiology
DOI: 10.1104/pp.16.01898
Access Rights: Open Access

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