Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling
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Tredwell, Greg; Aw, Rochelle; Edwards-Jones, Bryn; Leak, David J; Bundy, Jacob G
Description
Heterologous protein production in the yeast Pichia pastoris can be limited by biological responses to high expression levels; the unfolded protein response (UPR) is a key determinant of the success of protein production in this organism. Here, we used untargeted NMR metabolic profiling (metabolomics) of a number of different recombinant strains, carried out in a miniaturized format suitable for screening-level experiments. We identified a number of metabolites (from both cell extracts and...[Show more]
Collections | ANU Research Publications |
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Date published: | 2017 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/247704 |
Source: | Journal of Industrial Microbiology and Biotechnology |
DOI: | 10.1007/s10295-017-1904-5 |
Access Rights: | Open Access |
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01_Tredwell_Rapid_screening_of_cellular_2017.pdf | 525.27 kB | Adobe PDF |
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