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Rapid and Specific Detection of the Escherichia coli Sequence Type 648 Complex within Phylogroup F

Johnson, James R; Johnston, Brian D.; Gordon, David


The Escherichia coli sequence type 648 complex (STc648) is an emerging lineage within phylogroup F-formerly included within phylogroup D-that is associated with multidrug resistance. Here, we designed and validated a novel multiplex PCR-based assay for STc648 that took advantage of (i) four distinctive single-nucleotide polymorphisms in icd allele 96 and gyrB allele 87, two of the multilocus sequence typing alleles that define ST648; and (ii) the typical absence within STc648 of uidA, an E....[Show more]

CollectionsANU Research Publications
Date published: 2017
Type: Journal article
Source: Journal of Clinical Microbiology
DOI: 10.1128/JCM.01949-16


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