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Cell-specific localization of Na+ in roots of durum wheat and possible control points for salt exclusion

Huang, Cheng; Lauchli, Andre; James, Richard A; McCully, Margaret E; Munns, Rana Ellen

Description

Sodium exclusion from leaves is an important mechanism for salt tolerance in durum wheat. To characterize possible control points for Na+ exclusion, quantitative cryo-analytical scanning electron microscopy was used to determine cell-specific ion profiles across roots of two durum wheat genotypes with contrasting rates of Na+ transport from root to shoot grown in 50 mm NaCl. The Na+ concentration in Line 149 (low transport genotype) declined across the cortex, being highest in the epidermal and...[Show more]

dc.contributor.authorHuang, Cheng
dc.contributor.authorLauchli, Andre
dc.contributor.authorJames, Richard A
dc.contributor.authorMcCully, Margaret E
dc.contributor.authorMunns, Rana Ellen
dc.date.accessioned2015-12-07T22:42:55Z
dc.date.available2015-12-07T22:42:55Z
dc.identifier.issn0140-7791
dc.identifier.urihttp://hdl.handle.net/1885/24765
dc.description.abstractSodium exclusion from leaves is an important mechanism for salt tolerance in durum wheat. To characterize possible control points for Na+ exclusion, quantitative cryo-analytical scanning electron microscopy was used to determine cell-specific ion profiles across roots of two durum wheat genotypes with contrasting rates of Na+ transport from root to shoot grown in 50 mm NaCl. The Na+ concentration in Line 149 (low transport genotype) declined across the cortex, being highest in the epidermal and sub-epidermal cells (48 mm) and lowest in the inner cortical cells (22 mm). Na+ was high in the pericycle (85 mm) and low in the xylem parenchyma (34 mm). The Na+ profile in Tamaroi (high transport genotype) had a similar trend but with a high concentration (130 mm) in the xylem parenchyma. The K+ profiles were generally inverse to those of Na+. Chloride was only detected in the epidermis. These data suggest that the epidermal and cortical cells removed most of the Na+ and Cl - from the transpiration stream before it reached the endodermis, and that the endodermis is not the control point for salt uptake by the plant. The pericycle as well as the xylem parenchyma may be important in the control of net Na+ loading of the xylem.
dc.publisherBlackwell Publishing Ltd
dc.sourcePlant Cell and Environment
dc.subjectKeywords: chloride; potassium; sodium; sodium chloride; biological uptake; genotype; phloem; potassium; root; salinity tolerance; scanning electron microscopy; shoot growth; sodium; sodium chloride; transpiration; wheat; xylem; article; cryopreservation; drug effec Chloride; Endodermis; Epidermis; HKT transporter; Phloem; Salinity; Sodium; Xylem
dc.titleCell-specific localization of Na+ in roots of durum wheat and possible control points for salt exclusion
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume31
dc.date.issued2008
local.identifier.absfor060702 - Plant Cell and Molecular Biology
local.identifier.ariespublicationu4326120xPUB34
local.type.statusPublished Version
local.contributor.affiliationHuang, Cheng, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationLauchli, Andre, University of California
local.contributor.affiliationJames, Richard A, CSIRO Division of Plant Industry
local.contributor.affiliationMcCully, Margaret E, CSIRO Division of Plant Industry
local.contributor.affiliationMunns, Rana Ellen, CSIRO Division of Plant Industry
local.bibliographicCitation.startpage1565
local.bibliographicCitation.lastpage1574
local.identifier.doi10.1111/j.1365-3040.2008.01864.x
dc.date.updated2015-12-07T11:12:56Z
local.identifier.scopusID2-s2.0-53349160587
CollectionsANU Research Publications

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