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Multiple oligomeric forms of Escherichia coli DnaB helicase revealed by electrospray ionisation mass spectrometry

Watt, Stephen J; Urathamakul, Thitima; Schaeffer, Patrick; Williams, Neal; Sheil, Margaret; Dixon, Nicholas; Beck, Jennifer


The Escherichia coli DnaB protein (DnaB6) is the hexameric helicase that unwinds genomic DNA so it can be copied by the DNA replication machinery. Loading of the helicase onto DNA requires interactions of DnaB6 with six molecules of its loading partner protein, DnaC. Nano-electrospray ionisation mass spectrometry (nanoESI-MS) of mutant proteins was used to examine the roles of the residues Phe102 (F102) and Asp82 (D82) in the N-terminal domain of DnaB in the assembly of the hexamer. When the...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: Rapid Communications in Mass Spectrometry
DOI: 10.1002/rcm.2818


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