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Patterns of gene expression in developing embryos of Arabidopsis hybrids

Alonso-Peral, Maria M.; Trigueros, Marina; Sherman, Bjorg; Ying, Hua; Taylor, Jennifer; Peacock, William J.; Dennis, Elizabeth

Description

Hybrids between the Arabidopsis ecotypes C24 and Ler have high levels of hybrid vigour, or heterosis, in both biomass and seed yield. Heterosis can be detected throughout the development of the plant and in different tissues. We examined developing embryos and seeds of C24/Ler reciprocal hybrids with the aim of detecting the earliest time at which heterotic gene activity occurs. In the transcriptomes of 4-dap (days after pollination; dermatogen to globular) and 6-dap (heart) embryos from both...[Show more]

dc.contributor.authorAlonso-Peral, Maria M.
dc.contributor.authorTrigueros, Marina
dc.contributor.authorSherman, Bjorg
dc.contributor.authorYing, Hua
dc.contributor.authorTaylor, Jennifer
dc.contributor.authorPeacock, William J.
dc.contributor.authorDennis, Elizabeth
dc.date.accessioned2021-08-27T04:15:18Z
dc.identifier.issn0960-7412
dc.identifier.urihttp://hdl.handle.net/1885/245841
dc.description.abstractHybrids between the Arabidopsis ecotypes C24 and Ler have high levels of hybrid vigour, or heterosis, in both biomass and seed yield. Heterosis can be detected throughout the development of the plant and in different tissues. We examined developing embryos and seeds of C24/Ler reciprocal hybrids with the aim of detecting the earliest time at which heterotic gene activity occurs. In the transcriptomes of 4-dap (days after pollination; dermatogen to globular) and 6-dap (heart) embryos from both parents and hybrids, 95% of expressed genes were at the mid parent value (MPV) and 95% of the genes with single nucleotide polymorphisms between C24 and Ler retained the same relative allelic expression levels in the hybrids as existed in the parents. This included loci that had equivalent levels of transcription in the two parents, together with loci which had different levels of expression in the parents. Amongst the genes which did not have MPV expression levels in the hybrids (non-additively expressed genes), approximately 40 in the globular embryo stage and 89 in the heart embryo stage had altered levels of transcription in both reciprocal hybrids; these genes could contribute to the heterotic phenotype of the hybrid embryo. Many of the non-additively expressed genes had expression levels that were shifted towards maternal levels of transcription, and these differed in the reciprocal hybrids. Allelic expression analysis indicated that most genes with altered allelic contributions in the hybrids had an increase in the expression level of the hybrid's maternal allele. Consistent with the maternal pattern of gene expression, embryo and seed also show maternally influenced phenotypes
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherBlackwell Publishing Ltd
dc.rights© 2016 The Authors ; The Plant Journal © 2016 John Wiley & Sons Ltd
dc.sourceThe Plant Journal
dc.subjectArabidopsis thaliana
dc.subjectembryos
dc.subjecthybrids
dc.subjecttranscriptome
dc.titlePatterns of gene expression in developing embryos of Arabidopsis hybrids
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume89
dc.date.issued2017
local.identifier.absfor060408 - Genomics
local.identifier.ariespublicationa383154xPUB5224
local.publisher.urlhttps://www.wiley.com/en-gb
local.type.statusPublished Version
local.contributor.affiliationAlonso-Peral, Maria M., CSIRO
local.contributor.affiliationTrigueros, Marina, CSIRO
local.contributor.affiliationSherman, Bjorg, CSIRO
local.contributor.affiliationYing, Hua, College of Science, ANU
local.contributor.affiliationTaylor, Jennifer, CSIRO
local.contributor.affiliationPeacock, William J., CSIRO
local.contributor.affiliationDennis, Elizabeth, CSIRO
local.description.embargo2099-12-31
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage927
local.bibliographicCitation.lastpage939
local.identifier.doi10.1111/tpj.13432
local.identifier.absseo820299 - Horticultural Crops not elsewhere classified
dc.date.updated2020-11-23T10:55:36Z
local.identifier.scopusID2-s2.0-85012871833
local.identifier.thomsonID000395810800006
CollectionsANU Research Publications

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