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Non-intrusive assessment of photosystem II and photosystem I in whole coral tissues

Szabo, Milan; Larkum, A W D; Suggett, David J.; Vass, Imre; Sass, Laszlo; Osmond, Barry; Zavafer, Alonso; Ralph, Peter J; Chow, Wah Soon

Description

Reef building corals (phylum Cnidaria) harbor endosymbiotic dinoflagellate algae (genus Symbiodinium) that generate photosynthetic products to fuel their host's metabolism. Non-invasive techniques such as chlorophyll (Chl) fluorescence analyses of Photosystem II (PSII) have been widely used to estimate the photosynthetic performance of Symbiodinium in hospite. However, since the spatial origin of PSII chlorophyll fluorescence in coral tissues is uncertain, such signals give limited information...[Show more]

dc.contributor.authorSzabo, Milan
dc.contributor.authorLarkum, A W D
dc.contributor.authorSuggett, David J.
dc.contributor.authorVass, Imre
dc.contributor.authorSass, Laszlo
dc.contributor.authorOsmond, Barry
dc.contributor.authorZavafer, Alonso
dc.contributor.authorRalph, Peter J
dc.contributor.authorChow, Wah Soon
dc.date.accessioned2021-08-19T03:55:50Z
dc.date.available2021-08-19T03:55:50Z
dc.identifier.issn2296-7745
dc.identifier.urihttp://hdl.handle.net/1885/244040
dc.description.abstractReef building corals (phylum Cnidaria) harbor endosymbiotic dinoflagellate algae (genus Symbiodinium) that generate photosynthetic products to fuel their host's metabolism. Non-invasive techniques such as chlorophyll (Chl) fluorescence analyses of Photosystem II (PSII) have been widely used to estimate the photosynthetic performance of Symbiodinium in hospite. However, since the spatial origin of PSII chlorophyll fluorescence in coral tissues is uncertain, such signals give limited information on depth-integrated photosynthetic performance of the whole tissue. In contrast, detection of absorbance changes in the near infrared (NIR) region integrates signals from deeper tissue layers due to weak absorption and multiple scattering of NIR light. While extensively utilized in higher plants, NIR bio-optical techniques are seldom applied to corals. We have developed a non-intrusive measurement method to examine photochemistry of intact corals, based on redox kinetics of the primary electron donor in Photosystem I (P700) and chlorophyll fluorescence kinetics (Fast-Repetition Rate fluorometry, FRRf). Since the redox state of P700 depends on the operation of both PSI and PSII, important information can be obtained on the PSII-PSI intersystem electron transfer kinetics. Under moderate, sub-lethal heat stress treatments (33°C for ~20 min), the coral Pavona decussata exhibited down-regulation of PSII electron transfer kinetics, indicated by slower rates of electron transport from QA to plastoquinone (PQ) pool, and smaller relative size of oxidized PQ with concomitant decrease of a specifically-defined P700 kinetics area, which represents the active pool of PSII. The maximum quantum efficiency of PSII (Fv/Fm) and functional absorption cross-section of PSII (σPSII) remained unchanged. Based on the coordinated response of P700 parameters and PSII-PSI electron transport properties, we propose that simple P700 kinetics parameters as employed here serve as indicators of the integrity of PSII-PSI electron transfer dynamics in corals.
dc.description.sponsorshipThis work was supported by the award of Australian Research Council Discovery Project (ARC DP120101360) to WC and Future Fellowship (FT130100202) to DS. IV and LS were partly supported by a grant from the Hungarian National Research, Development and Innovation Office (NN110960)
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherFrontiers Research Foundation
dc.rightsCopyright © 2017 Szabó, Larkum, Suggett, Vass, Sass, Osmond, Zavafer, Ralph and Chow.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceFrontiers in Marine Science
dc.subjectcorals
dc.subjectphotosynthesis
dc.subjectP700
dc.subjectchlorophyll fluorescence
dc.subjectheat stress
dc.titleNon-intrusive assessment of photosystem II and photosystem I in whole coral tissues
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume4
dc.date.issued2017
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationa383154xPUB8352
local.publisher.urlhttp://frontiersin.org/Marine_Science
local.type.statusPublished Version
local.contributor.affiliationSzabo, Milan, College of Science, ANU
local.contributor.affiliationLarkum, A W D, University of Technology Sydney
local.contributor.affiliationSuggett, David J., University of Technology, Sydney
local.contributor.affiliationVass, Imre, Biological Research Center of the Hungarian Academy of Sciences,
local.contributor.affiliationSass, Laszlo, Biological Research Center of the Hungarian Academy of Sciences
local.contributor.affiliationOsmond, C Barry, College of Science, ANU
local.contributor.affiliationZavaleta Fernandez de Cordova, Alonso, College of Science, ANU
local.contributor.affiliationRalph, Peter J, University of Technology Sydney
local.contributor.affiliationChow, Wah, College of Science, ANU
dc.relationhttp://purl.org/au-research/grants/arc/FT130100202
local.bibliographicCitation.issueAUG
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage12
local.identifier.doi10.3389/fmars.2017.00269
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2020-11-23T10:53:08Z
local.identifier.scopusID2-s2.0-85027708535
dcterms.accessRightsOpen Access
dc.provenanceThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.licenseCreative Commons Attribution License (CC BY)
CollectionsANU Research Publications

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