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Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modeling and Assessment

Mitchell, Joshua; Zhang, William; Herde, Michel K.; Henneberger, Christian; Janovjak, Harald; O'Mara, Megan; Jackson, Colin


Biosensors that exploit Förster resonance energy transfer (FRET) can be used to visualize biological and physiological processes and are capable of providing detailed information in both spatial and temporal dimensions. In a FRET-based biosensor, substrate binding is associated with a change in the relative positions of two fluorophores, leading to a change in FRET efficiency that may be observed in the fluorescence spectrum. As a result, their design requires a ligand-binding protein that...[Show more]

CollectionsANU Research Publications
Date published: 2017
Type: Book chapter
Book Title: Synthetic Protein Switches : Methods and Protocols
DOI: 10.1007/978-1-4939-6940-1_6


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