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Measuring CO2 and HCO3 - permeabilities of isolated chloroplasts using a MIMS-18O approach

Tolleter, Dimitri; Chochois, Vincent; Poire-Lassus, Richard; Price, Graeme; Badger, Murray


To support photosynthetic CO2 fixation by Rubisco, the chloroplast must be fed with inorganic carbon in the form of CO2 or bicarbonate. However, the mechanisms allowing the rapid passage of this gas and this charged molecule through the bounding membranes of the chloroplast envelope are not yet completely elucidated. We describe here a method allowing us to measure the permeability of these two molecules through the chloroplast envelope using a membrane inlet mass spectrometer and 18O-labelled...[Show more]

CollectionsANU Research Publications
Date published: 2017
Type: Journal article
Source: Journal of Experimental Botany
DOI: 10.1093/jxb/erx188
Access Rights: Open Access


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