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High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope

Li, Yongxiao; Montague, Samantha; Bruestle, Anne; He, Xuefei; Gillespie, Catherine; Gaus, Katharina; Gardiner, Elizabeth; Lee, Steve

Description

In this study, we introduce two key improvements that overcome limitations of existing polygon scanning microscopes while maintaining high spatial and temporal imaging resolution over large field of view (FOV). First, we proposed a simple and straightforward means to control the scanning angle of the polygon mirror to carry out photomanipulation without resorting to high speed optical modulators. Second, we devised a flexible data sampling method directly leading to higher image contrast by...[Show more]

dc.contributor.authorLi, Yongxiao
dc.contributor.authorMontague, Samantha
dc.contributor.authorBruestle, Anne
dc.contributor.authorHe, Xuefei
dc.contributor.authorGillespie, Catherine
dc.contributor.authorGaus, Katharina
dc.contributor.authorGardiner, Elizabeth
dc.contributor.authorLee, Steve
dc.date.accessioned2021-07-26T01:29:56Z
dc.date.available2021-07-26T01:29:56Z
dc.identifier.citationLi Y, Montague SJ, Brüstle A, et al. High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope. J. Biophotonics. 2018;e201700341. https://doi.org/10.1002/jbio.201700341
dc.identifier.issn1864-063X
dc.identifier.urihttp://hdl.handle.net/1885/241071
dc.description.abstractIn this study, we introduce two key improvements that overcome limitations of existing polygon scanning microscopes while maintaining high spatial and temporal imaging resolution over large field of view (FOV). First, we proposed a simple and straightforward means to control the scanning angle of the polygon mirror to carry out photomanipulation without resorting to high speed optical modulators. Second, we devised a flexible data sampling method directly leading to higher image contrast by over 2‐fold and digital images with 100 megapixels (10 240 × 10 240) per frame at 0.25 Hz. This generates sub‐diffraction limited pixels (60 nm per pixels over the FOV of 512 μm) which increases the degrees of freedom to extract signals computationally. The unique combined optical and digital control recorded fine fluorescence recovery after localized photobleaching (r ~10 μm) within fluorescent giant unilamellar vesicles and micro‐vascular dynamics after laser‐induced injury during thrombus formation in vivo. These new improvements expand the quantitative biological‐imaging capacity of any polygon scanning microscope system.
dc.description.sponsorshipAustralian Research Council, Grant/Award Number: DE160100843
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherWiley-VCH Verlag GMBH
dc.rights© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.sourceJournal of Biophotonics
dc.subjectmultiphoton microscopy
dc.subjectoptical design and fabrication
dc.subjectscanning microscopy
dc.titleHigh contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume11
dcterms.dateAccepted2018-02-26
dc.date.issued2018-07-08
local.identifier.absfor060106 - Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
local.identifier.absfor060110 - Receptors and Membrane Biology
local.identifier.absfor090303 - Biomedical Instrumentation
local.identifier.ariespublicationu1029610xPUB128
local.publisher.urlhttps://onlinelibrary.wiley.com/
local.type.statusAccepted Version
local.contributor.affiliationLi, Yongxiao, College of Engineering and Computer Science, ANU
local.contributor.affiliationMontague, Samantha, College of Health and Medicine, ANU
local.contributor.affiliationBruestle, Anne, College of Health and Medicine, ANU
local.contributor.affiliationHe, Xuefei, College of Engineering and Computer Science, ANU
local.contributor.affiliationGillespie, Catherine, College of Health and Medicine, ANU
local.contributor.affiliationGaus, Katharina, University of New South Wales
local.contributor.affiliationGardiner, Elizabeth, College of Health and Medicine, ANU
local.contributor.affiliationLee, Steve, College of Engineering and Computer Science, ANU
dc.relationhttp://purl.org/au-research/grants/arc/DE160100843
local.bibliographicCitation.issue7
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage12
local.identifier.doi10.1002/jbio.201700341
local.identifier.absseo920103 - Cardiovascular System and Diseases
local.identifier.absseo920101 - Blood Disorders
dc.date.updated2020-11-23T11:43:20Z
local.identifier.scopusID2-s2.0-85044921926
dcterms.accessRightsOpen Access
dc.provenancehttps://v2.sherpa.ac.uk/id/publication/1776..."Author accepted manuscript can be made open access on non-commercial institutional repository after 12 month embargo" from SHERPA/RoMEO site (as at 26.7.2021).
CollectionsANU Research Publications

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