High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope
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Li, Yongxiao; Montague, Samantha; Bruestle, Anne; He, Xuefei; Gillespie, Catherine; Gaus, Katharina; Gardiner, Elizabeth; Lee, Steve
Description
In this study, we introduce two key improvements that overcome limitations of existing polygon scanning microscopes while maintaining high spatial and temporal imaging resolution over large field of view (FOV). First, we proposed a simple and straightforward means to control the scanning angle of the polygon mirror to carry out photomanipulation without resorting to high speed optical modulators. Second, we devised a flexible data sampling method directly leading to higher image contrast by...[Show more]
dc.contributor.author | Li, Yongxiao | |
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dc.contributor.author | Montague, Samantha | |
dc.contributor.author | Bruestle, Anne | |
dc.contributor.author | He, Xuefei | |
dc.contributor.author | Gillespie, Catherine | |
dc.contributor.author | Gaus, Katharina | |
dc.contributor.author | Gardiner, Elizabeth | |
dc.contributor.author | Lee, Steve | |
dc.date.accessioned | 2021-07-26T01:29:56Z | |
dc.date.available | 2021-07-26T01:29:56Z | |
dc.identifier.citation | Li Y, Montague SJ, Brüstle A, et al. High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope. J. Biophotonics. 2018;e201700341. https://doi.org/10.1002/jbio.201700341 | |
dc.identifier.issn | 1864-063X | |
dc.identifier.uri | http://hdl.handle.net/1885/241071 | |
dc.description.abstract | In this study, we introduce two key improvements that overcome limitations of existing polygon scanning microscopes while maintaining high spatial and temporal imaging resolution over large field of view (FOV). First, we proposed a simple and straightforward means to control the scanning angle of the polygon mirror to carry out photomanipulation without resorting to high speed optical modulators. Second, we devised a flexible data sampling method directly leading to higher image contrast by over 2‐fold and digital images with 100 megapixels (10 240 × 10 240) per frame at 0.25 Hz. This generates sub‐diffraction limited pixels (60 nm per pixels over the FOV of 512 μm) which increases the degrees of freedom to extract signals computationally. The unique combined optical and digital control recorded fine fluorescence recovery after localized photobleaching (r ~10 μm) within fluorescent giant unilamellar vesicles and micro‐vascular dynamics after laser‐induced injury during thrombus formation in vivo. These new improvements expand the quantitative biological‐imaging capacity of any polygon scanning microscope system. | |
dc.description.sponsorship | Australian Research Council, Grant/Award Number: DE160100843 | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_AU | |
dc.publisher | Wiley-VCH Verlag GMBH | |
dc.rights | © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | |
dc.source | Journal of Biophotonics | |
dc.subject | multiphoton microscopy | |
dc.subject | optical design and fabrication | |
dc.subject | scanning microscopy | |
dc.title | High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 11 | |
dcterms.dateAccepted | 2018-02-26 | |
dc.date.issued | 2018-07-08 | |
local.identifier.absfor | 060106 - Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) | |
local.identifier.absfor | 060110 - Receptors and Membrane Biology | |
local.identifier.absfor | 090303 - Biomedical Instrumentation | |
local.identifier.ariespublication | u1029610xPUB128 | |
local.publisher.url | https://onlinelibrary.wiley.com/ | |
local.type.status | Accepted Version | |
local.contributor.affiliation | Li, Yongxiao, College of Engineering and Computer Science, ANU | |
local.contributor.affiliation | Montague, Samantha, College of Health and Medicine, ANU | |
local.contributor.affiliation | Bruestle, Anne, College of Health and Medicine, ANU | |
local.contributor.affiliation | He, Xuefei, College of Engineering and Computer Science, ANU | |
local.contributor.affiliation | Gillespie, Catherine, College of Health and Medicine, ANU | |
local.contributor.affiliation | Gaus, Katharina, University of New South Wales | |
local.contributor.affiliation | Gardiner, Elizabeth, College of Health and Medicine, ANU | |
local.contributor.affiliation | Lee, Steve, College of Engineering and Computer Science, ANU | |
dc.relation | http://purl.org/au-research/grants/arc/DE160100843 | |
local.bibliographicCitation.issue | 7 | |
local.bibliographicCitation.startpage | 1 | |
local.bibliographicCitation.lastpage | 12 | |
local.identifier.doi | 10.1002/jbio.201700341 | |
local.identifier.absseo | 920103 - Cardiovascular System and Diseases | |
local.identifier.absseo | 920101 - Blood Disorders | |
dc.date.updated | 2020-11-23T11:43:20Z | |
local.identifier.scopusID | 2-s2.0-85044921926 | |
dcterms.accessRights | Open Access | |
dc.provenance | https://v2.sherpa.ac.uk/id/publication/1776..."Author accepted manuscript can be made open access on non-commercial institutional repository after 12 month embargo" from SHERPA/RoMEO site (as at 26.7.2021). | |
Collections | ANU Research Publications |
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File | Description | Size | Format | Image |
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jbio.201700341.pdf | Author Accepted Manuscript | 10.09 MB | Adobe PDF |
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