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High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope

Li, Yongxiao; Montague, Samantha; Bruestle, Anne; He, Xuefei; Gillespie, Catherine; Gaus, Katharina; Gardiner, Elizabeth; Lee, Steve


In this study, we introduce two key improvements that overcome limitations of existing polygon scanning microscopes while maintaining high spatial and temporal imaging resolution over large field of view (FOV). First, we proposed a simple and straightforward means to control the scanning angle of the polygon mirror to carry out photomanipulation without resorting to high speed optical modulators. Second, we devised a flexible data sampling method directly leading to higher image contrast by...[Show more]

CollectionsANU Research Publications
Date published: 2018-07-08
Type: Journal article
Source: Journal of Biophotonics
DOI: 10.1002/jbio.201700341
Access Rights: Open Access


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