Skip navigation
Skip navigation

High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope

Li, Yongxiao; Montague, Samantha; Bruestle, Anne; He, Xuefei; Gillespie, Catherine; Gaus, Katharina; Gardiner, Elizabeth; Lee, Steve

Description

In this study, we introduce two key improvements that overcome limitations of existing polygon scanning microscopes while maintaining high spatial and temporal imaging resolution over large field of view (FOV). First, we proposed a simple and straightforward means to control the scanning angle of the polygon mirror to carry out photomanipulation without resorting to high speed optical modulators. Second, we devised a flexible data sampling method directly leading to higher image contrast by...[Show more]

CollectionsANU Research Publications
Date published: 2018-07-08
Type: Journal article
URI: http://hdl.handle.net/1885/241071
Source: Journal of Biophotonics
DOI: 10.1002/jbio.201700341
Access Rights: Open Access

Download

File Description SizeFormat Image
jbio.201700341.pdfAuthor Accepted Manuscript10.09 MBAdobe PDFThumbnail


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator