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Impact of the c-MybE308G mutation on mouse myelopoiesis and dendritic cell development

Papathanasiou, Panagiotis; Petvises, Sawang; Hey, Ying; Perkins, Andrew C.; O'Neill, Helen C

Description

Booreana mice carrying the c-Myb308G point mutation were analyzed to determine changes in early hematopoiesis in the bone marrow and among mature cells in the periphery. This point mutation led to increased numbers of early hematopoietic stem and progenitor cells (HSPCs), with a subsequent reduction in the development of B cells, erythroid cells, and neutrophils, and increased numbers of myeloid cells and granulocytes. Myelopoiesis was further investigated by way of particular subsets affected....[Show more]

dc.contributor.authorPapathanasiou, Panagiotis
dc.contributor.authorPetvises, Sawang
dc.contributor.authorHey, Ying
dc.contributor.authorPerkins, Andrew C.
dc.contributor.authorO'Neill, Helen C
dc.date.accessioned2021-07-08T00:00:03Z
dc.date.available2021-07-08T00:00:03Z
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1885/238608
dc.description.abstractBooreana mice carrying the c-Myb308G point mutation were analyzed to determine changes in early hematopoiesis in the bone marrow and among mature cells in the periphery. This point mutation led to increased numbers of early hematopoietic stem and progenitor cells (HSPCs), with a subsequent reduction in the development of B cells, erythroid cells, and neutrophils, and increased numbers of myeloid cells and granulocytes. Myelopoiesis was further investigated by way of particular subsets affected. A specific question addressed whether booreana mice contained increased numbers of dendritic-like cells (L-DC subset) recently identified in the spleen, since L-DCs arise in vitro by direct differentiation from HSPCs co-cultured over splenic stroma. The non-lethal c-Myb mutation in booreana mice was associated with significantly lower representation of splenic CD8- conventional dendritic cells (cDCs), inflammatory monocytes, and neutrophils compared to wild-type mice. This result confirmed the bone marrow origin of progenitors for these subsets since c-Myb is essential for their development. Production of L-DCs and resident monocytes was not affected by the c-MybE308G mutation. These subsets may derive from different progenitors than those in bone marrow, and are potentially established in the spleen during embryogenesis. An alternative explanation may be needed for why there was no change in CD8+ cDCs in booreana spleen since these cells are known to derive from common dendritic progenitors in bone marrow.
dc.description.sponsorshipThis work was supported by a project grant #585443 to HO from the National Health and Medical Research Council (NHMRC) of Australia. PP was supported by an NHMRC CJ Martin Fellowship. YH was supported by a postgraduate scholarship from the Australian National University
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherPublic Library of Science
dc.rights© 2017 Papathanasiou et al.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePLOS ONE (Public Library of Science)
dc.titleImpact of the c-MybE308G mutation on mouse myelopoiesis and dendritic cell development
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume12
dc.date.issued2017
local.identifier.absfor110704 - Cellular Immunology
local.identifier.absfor110799 - Immunology not elsewhere classified
local.identifier.ariespublicationa383154xPUB5777
local.publisher.urlhttp://www.plosone.org/
local.type.statusPublished Version
local.contributor.affiliationPapathanasiou, Panagiotis, College of Science, ANU
local.contributor.affiliationPetvises, Sawang, College of Science, ANU
local.contributor.affiliationHey, Ying, College of Science, ANU
local.contributor.affiliationPerkins, Andrew C., University of Queensland
local.contributor.affiliationO'Neill, Helen C, Bond University
dc.relationhttp://purl.org/au-research/grants/nhmrc/585443
local.bibliographicCitation.issue4
local.bibliographicCitation.startpagee176345
local.bibliographicCitation.lastpagee176345
local.identifier.doi10.1371/journal.pone.0176345
local.identifier.absseo920108 - Immune System and Allergy
dc.date.updated2020-11-23T10:39:49Z
local.identifier.scopusID2-s2.0-85018179427
local.identifier.thomsonID000400309200048
dcterms.accessRightsOpen Access
dc.provenanceThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.licenseCreative Commons Attribution License
CollectionsANU Research Publications

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