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Fungal phytopathogens encode functional homologues of plant rapid alkalinisation factor (RALF) peptides

Thynne, Elisha; Saur, Isabel ML; Simbaqueba, Jaime; Ogilvie, Huw; Gonzalez Cendales, Yvonne; Mead, Oliver; Taranto, Adam; Catanzariti, Ann-Maree; McDonald, Megan; Schwessinger, Benjamin; Jones, David; Rathjen, John; Solomon, Peter

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In this paper we describe the presence of genes encoding close homologues of an endogenous plant peptide, rapid alkalinisation factor (RALF), within the genomes of 26 species of phytopathogenic fungi. Members of the RALF family are key growth factors in plants, and the sequence of the RALF active region is well conserved between the plant and fungal proteins. RALF1-like sequences were observed in most cases; however, RALF27-like sequences were present in the Sphaerulina musiva and Septoria...[Show more]

dc.contributor.authorThynne, Elisha
dc.contributor.authorSaur, Isabel ML
dc.contributor.authorSimbaqueba, Jaime
dc.contributor.authorOgilvie, Huw
dc.contributor.authorGonzalez Cendales, Yvonne
dc.contributor.authorMead, Oliver
dc.contributor.authorTaranto, Adam
dc.contributor.authorCatanzariti, Ann-Maree
dc.contributor.authorMcDonald, Megan
dc.contributor.authorSchwessinger, Benjamin
dc.contributor.authorJones, David
dc.contributor.authorRathjen, John
dc.contributor.authorSolomon, Peter
dc.date.accessioned2021-06-28T02:20:49Z
dc.date.available2021-06-28T02:20:49Z
dc.identifier.issn1464-6722
dc.identifier.urihttp://hdl.handle.net/1885/238276
dc.description.abstractIn this paper we describe the presence of genes encoding close homologues of an endogenous plant peptide, rapid alkalinisation factor (RALF), within the genomes of 26 species of phytopathogenic fungi. Members of the RALF family are key growth factors in plants, and the sequence of the RALF active region is well conserved between the plant and fungal proteins. RALF1-like sequences were observed in most cases; however, RALF27-like sequences were present in the Sphaerulina musiva and Septoria populicola genomes. These two species are pathogens of poplar and interestingly, the closest relative to their respective RALF genes is a poplar RALF27-like sequence. RALF peptides control cellular expansion during plant development, but were originally defined based on their ability to induce rapid alkalinisation in tobacco cell cultures. To test whether the fungal RALF peptides were biologically active in plants, we synthesized RALF peptides corresponding to those encoded by two sequenced genomes of the tomato pathogen Fusarium oxysporum f. sp. lycopersici. One of these peptides inhibited the growth of tomato seedlings and elicited responses in tomato and Nicotiana benthamiana typical of endogenous plant RALF peptides (ROS burst, induced alkalinisation and MAP kinas activation). Gene expression analysis confirmed that a RALF-encoding gene in Fusarium oxysporum f. sp. lycopersici was expressed during infection on tomato. However a subsequent reverse genetics approach revealed that the RALF peptide was not required by Fusarium oxysporum f. sp. lycopersici for infection on tomato roots. This study has demonstrated the presence of functionally active RALF peptides encoded within phytopathogens that harbour an as yet undetermined role in the plant-pathogen interactions. This article is protected by copyright. All rights reserved.
dc.description.sponsorshipThe authors would like to thank Dr Markus Albert for technical advice on the alkalinization assay. PSS is an Australian Research Council Future Fellow (FT110100698). BS is supported by an Australian Research Council Discovery Early Career Award (DE150101897). ET is supported by an Australian Postgraduate Award and a Grains Research and Development Corporation Scholarship.
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherBlackwell Publishing Ltd
dc.rights© 2016 BSPP AND JOHN WILEY & SONS LTD
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceMolecular Plant Pathology
dc.subjectFusarium
dc.subjectphytopathogen effectors
dc.subjectrapid alkalinization factor (RALF)
dc.titleFungal phytopathogens encode functional homologues of plant rapid alkalinisation factor (RALF) peptides
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume18
dc.date.issued2017
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationu4956746xPUB590
local.publisher.urlhttps://www.wiley.com/en-gb
local.type.statusPublished Version
local.contributor.affiliationThynne, Elisha, College of Science, ANU
local.contributor.affiliationSaur, Isabel, College of Science, ANU
local.contributor.affiliationSimbaqueba, Jaime, College of Science, ANU
local.contributor.affiliationOgilvie, Huw, College of Science, ANU
local.contributor.affiliationGonzalez Cendales, Yvonne, College of Health and Medicine, ANU
local.contributor.affiliationMead, Oliver, College of Science, ANU
local.contributor.affiliationTaranto, Adam, College of Science, ANU
local.contributor.affiliationCatanzariti, Ann-Maree, College of Science, ANU
local.contributor.affiliationMcDonald, Megan, College of Science, ANU
local.contributor.affiliationSchwessinger, Benjamin, College of Science, ANU
local.contributor.affiliationJones, David, College of Science, ANU
local.contributor.affiliationRathjen, John, College of Science, ANU
local.contributor.affiliationSolomon, Peter, College of Science, ANU
dc.relationhttp://purl.org/au-research/grants/arc/FT110100698
dc.relationhttp://purl.org/au-research/grants/arc/DE150101897
local.bibliographicCitation.issue6
local.bibliographicCitation.startpage811
local.bibliographicCitation.lastpage824
local.identifier.doi10.1111/mpp.12444
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2020-11-23T10:34:45Z
local.identifier.scopusID2-s2.0-84982920644
local.identifier.thomsonID000405096600005
dcterms.accessRightsOpen Access
dc.provenancehttps://v2.sherpa.ac.uk/id/publication/7107..."The Published Version can be archived in any website" from SHERPA/RoMEO site (as at 28/06/2021).
dc.rights.licenseCC BY-NC-ND
CollectionsANU Research Publications

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