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Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors

Clifton, Benjamin; Whitfield, Jason; Sanchez-Romero, Immaculada; Herde, Michel; Henneberger, Christian; Janovjak, Harald; Jackson, Colin


Small molecule biosensors based on Förster resonance energy transfer (FRET) enable small molecule signaling to be monitored with high spatial and temporal resolution in complex cellular environments. FRET sensors can be constructed by fusing a pair of fluorescent proteins to a suitable recognition domain, such as a member of the solute-binding protein (SBP) superfamily. However, naturally occurring SBPs may be unsuitable for incorporation into FRET sensors due to their low thermostability,...[Show more]

CollectionsANU Research Publications
Date published: 2017
Type: Book chapter
Book Title: Synthetic Protein Switches : Methods and Protocols
DOI: 10.1007/978-1-4939-6940-1_5


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