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An environmental DNA assay for detecting Macquarie perch, Macquaria australasica

Piggott, Maxine

Description

Macquarie perch (Macquaria australasica) are an endangered freshwater fish found in the Murray-Darling Basin, Australia. I developed a species-specific assay to detect Macquarie perch DNA in environmental samples. The assay is highly specific to detect Macquarie perch, and produced no amplification from 18 co-distributed species. Testing the assay on environmental water samples demonstrated positive detections where Macquarie perch are known to be present in low and moderate density.

dc.contributor.authorPiggott, Maxine
dc.date.accessioned2021-05-06T23:52:39Z
dc.identifier.issn1877-7252
dc.identifier.urihttp://hdl.handle.net/1885/232513
dc.description.abstractMacquarie perch (Macquaria australasica) are an endangered freshwater fish found in the Murray-Darling Basin, Australia. I developed a species-specific assay to detect Macquarie perch DNA in environmental samples. The assay is highly specific to detect Macquarie perch, and produced no amplification from 18 co-distributed species. Testing the assay on environmental water samples demonstrated positive detections where Macquarie perch are known to be present in low and moderate density.
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherSpringer
dc.rights© Springer Science+Business Media Dordrecht 2017
dc.sourceConservation Genetics Resources
dc.source.urihttps://link.springer.com/article/10.1007%2Fs12686-016-0666-0
dc.subjecteDNA
dc.subjectEnvironmental DNA
dc.subjectMacquarie perch
dc.subjectMacquaria australasica
dc.subjectqPCR
dc.titleAn environmental DNA assay for detecting Macquarie perch, Macquaria australasica
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume9
dc.date.issued2017
local.identifier.absfor060201 - Behavioural Ecology
local.identifier.ariespublicationu9511635xPUB1670
local.publisher.urlhttps://link.springer.com
local.type.statusPublished Version
local.contributor.affiliationPiggott, Maxine, College of Science, ANU
local.description.embargo2099-12-31
dc.relationhttp://purl.org/au-research/grants/arc/DE130100777
local.bibliographicCitation.issue2
local.bibliographicCitation.startpage257
local.bibliographicCitation.lastpage259
local.identifier.doi10.1007/s12686-016-0666-0
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2020-11-23T10:11:01Z
local.identifier.scopusID2-s2.0-85027887857
local.identifier.thomsonID000401507200022
CollectionsANU Research Publications

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