Identification of essential loops and residues of glucosyltransferase V (GtrV) of Shigella Flexneri
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Korres, Haralambos; Verma, Naresh
Description
Lipopolysaccharide (LPS), particularly the O-antigen component, is one of many virulence determinants necessary for Shigella flexneri pathogenesis. O-antigen modification is mediated by glucosyltransferase (gtr) genes encoded by temperate serotype-converting bacteriophages. The gtrV and gtrX genes encode the GtrV and GtrX glucosyltransferases, respectively. These are integral membrane proteins, which catalyze the transfer of a glucosyl residue via an α1,3 linkage to rhamnose II and rhamnose I...[Show more]
dc.contributor.author | Korres, Haralambos | |
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dc.contributor.author | Verma, Naresh | |
dc.date.accessioned | 2015-12-07T22:33:11Z | |
dc.identifier.issn | 0968-7688 | |
dc.identifier.uri | http://hdl.handle.net/1885/23143 | |
dc.description.abstract | Lipopolysaccharide (LPS), particularly the O-antigen component, is one of many virulence determinants necessary for Shigella flexneri pathogenesis. O-antigen modification is mediated by glucosyltransferase (gtr) genes encoded by temperate serotype-converting bacteriophages. The gtrV and gtrX genes encode the GtrV and GtrX glucosyltransferases, respectively. These are integral membrane proteins, which catalyze the transfer of a glucosyl residue via an α1,3 linkage to rhamnose II and rhamnose I of the O-antigen unit. This mediates conversion of S. flexneri serotype Y to serotype 5a and X, respectively. Essential regions in the topology of GtrV protein were identified by in vivo recombination and a PCR-mediated approach. A series of GtrX-GtrV and GtrV-GtrX chimeric proteins were constructed based on the fact that GtrV and GtrX share sequence similarity. Analysis of their respective serotype conversion abilities led to the identification of two important periplasmic loops: loops No 2 and No 10 located in the N- and C-termini, respectively. Within these two loops, three conserved motifs were identified; two in loop No 2 and one in loop No 10. These conserved motifs contain acidic residues which were shown to be critical for GtrV function. | |
dc.publisher | Taylor & Francis Group | |
dc.source | Molecular Membrane Biology | |
dc.subject | Keywords: chimeric protein; glucosyltransferase; grtv enzyme; grtx enzyme; unclassified drug; amino terminal sequence; article; carboxy terminal sequence; controlled study; enzyme analysis; homologous recombination; in vivo study; nonhuman; polymerase chain reactio Bacteriophage; Chimeras; Dual reporter; Glucosyltransferase; Shigella flexneri | |
dc.title | Identification of essential loops and residues of glucosyltransferase V (GtrV) of Shigella Flexneri | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 23 | |
dc.date.issued | 2006 | |
local.identifier.absfor | 060501 - Bacteriology | |
local.identifier.ariespublication | u4325460xPUB25 | |
local.type.status | Published Version | |
local.contributor.affiliation | Korres, Haralambos, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Verma, Naresh, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 5 | |
local.bibliographicCitation.startpage | 407 | |
local.bibliographicCitation.lastpage | 419 | |
local.identifier.doi | 10.1080/09687860600849853 | |
dc.date.updated | 2015-12-07T10:28:35Z | |
local.identifier.scopusID | 2-s2.0-33750284420 | |
Collections | ANU Research Publications |
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