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Reassembling haplotypes in a mixture of pooled amplicons when the relative concentrations are known: A proof-of-concept study on the efficient design of nextgeneration sequencing strategies

Ranjard, Louis; Wong, Thomas; Rodrigo, Allen


Next-generation sequencing can be costly and labour intensive. Usually, the sequencing cost per sample is reduced by pooling amplified DNA = amplicons) derived from different individuals on the same sequencing lane. Barcodes unique to each amplicon permit short-read sequences to be assigned appropriately. However, the cost of the library preparation increases with the number of barcodes used. We propose an alternative to barcoding: by using different known proportions of individually-derived...[Show more]

CollectionsANU Research Publications
Date published: 2018-04-05
Type: Journal article
Source: PLOS ONE (Public Library of Science)
DOI: 10.1371/journal.pone.0195090
Access Rights: Open Access


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