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Ablation of the ASCT2 (SLC1A5) gene encoding a neutral amino acid transporter reveals transporter plasticity and redundancy in cancer cells

Broer, Angelika; Gauthier-Coles, Gregory; Rahimi, Farid; van Geldermalsen, Michelle; Dorsch, Dieter; Wegener, Ansgar; Holst, Jeff; Broer, Stefan


The neutral amino acid transporter solute carrier family 1 member 5 (SLC1A5 or ASCT2) is overexpressed in many cancers. To identify its roles in tumors, we employed 143B osteosarcoma cells and HCC1806 triple-negative breast cancer cells with or without ASCT2 deletion. ASCT2ko 143B cells grew well in standard culture media, but ASCT2 was required for optimal growth at < 0.5 mM glutamine, with tumor spheroid growth and monolayer migration of 143B ASCT2ko cells being strongly impaired at lower...[Show more]

CollectionsANU Research Publications
Date published: 2019-01-11
Type: Journal article
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.RA118.006378


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