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Directed Evolution of an Improved Rubisco; In Vitro Analyses to Decipher Fact from Fiction

Zhou, Yu; Whitney, Spencer


Inaccuracies in biochemically characterizing the amount and CO2-fixing properties of the photosynthetic enzyme Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase continue to hamper an accurate evaluation of Rubisco mutants selected by directed evolution. Here, we outline an analytical pipeline for accurately quantifying Rubisco content and kinetics that averts the misinterpretation of directed evolution outcomes. Our study utilizes a new T7-promoter regulated Rubisco Dependent Escherichia...[Show more]

CollectionsANU Research Publications
Date published: 2019
Type: Journal article
Source: International Journal of Molecular Sciences
DOI: 10.3390/ijms20205019
Access Rights: Open Access


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