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Backbone assignment of fully protonated solid proteins by 1 H detection and ultrafast magic-angle-spinning NMR spectroscopy

Marchetti, Alessandro; Jehle, Stefan; Felletti, Michele; Knight, Michael J.; Wang, Yao; Xu, Zhi-Qiang; Park, Ah Young; Lesage, Anne; Emsley, Lyndon; Dixon, Nicholas Edward; Pintacuda, Guido; Otting, Gottfried

Description

Narrow 1H NMR linewidths can be obtained for fully protonated protein samples in the solid state by using ultrafast magic-angle spinning (60 kHz). Medium-size microcrystalline and noncrystalline proteins can be analyzed without any need for deuteration of the protein sample. This approach provides assignments of the backbone 1H, 15N, 13C α, and 13CO resonances and yields information about 1H-1H proximities.

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
URI: http://hdl.handle.net/1885/22428
Source: Angewandte Chemie International Edition
DOI: 10.1002/anie.201203124

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