Draft Genome Resource for Macrophomina phaseolina Associated With Charcoal Rot in Sorghum
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Purushotham, Neeraj; Jones, Ashley; Poudel, Barsha; Nasim, Jamila; Adorada, Dante; Sparks, Adam; Schwessinger, Benjamin; Vaghefi, Niloofar
Description
Macrophomina phaseolina is a soil-borne phytopathogenic fungus that causes charcoal rot in several plant species, including sorghum. We constructed a draft genome of M. phaseolina isolate BRIP 70780a from sorghum, using long-read native DNA from MinION sequencing, which was error-corrected using short-read Illumina MiSeq reads. The draft genome, consisting of 22 contigs with an N50 of 4,257,441 bp, 99.3% complete benchmarking universal single-copy orthologs, and 14,471 genes, is a valuable...[Show more]
dc.contributor.author | Purushotham, Neeraj | |
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dc.contributor.author | Jones, Ashley | |
dc.contributor.author | Poudel, Barsha | |
dc.contributor.author | Nasim, Jamila | |
dc.contributor.author | Adorada, Dante | |
dc.contributor.author | Sparks, Adam | |
dc.contributor.author | Schwessinger, Benjamin | |
dc.contributor.author | Vaghefi, Niloofar | |
dc.date.accessioned | 2021-02-22T23:04:36Z | |
dc.identifier.issn | 0894-0282 | |
dc.identifier.uri | http://hdl.handle.net/1885/224127 | |
dc.description.abstract | Macrophomina phaseolina is a soil-borne phytopathogenic fungus that causes charcoal rot in several plant species, including sorghum. We constructed a draft genome of M. phaseolina isolate BRIP 70780a from sorghum, using long-read native DNA from MinION sequencing, which was error-corrected using short-read Illumina MiSeq reads. The draft genome, consisting of 22 contigs with an N50 of 4,257,441 bp, 99.3% complete benchmarking universal single-copy orthologs, and 14,471 genes, is a valuable resource to aid future studies in population genomics and molecular diagnostic marker development for rapid detection of the pathogen | |
dc.description.sponsorship | This research was funded and supported by the Grains Research and Development Corporation (projects GRDC 9176684, DAQ00194, and DAQ00186), Australia. | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_AU | |
dc.publisher | American Phytopathological Society | |
dc.rights | © 2020 The American Phytopathological Society | |
dc.source | Molecular Plant-Microbe Interactions (MPMI) | |
dc.subject | Macrophomina phaseolina | |
dc.subject | Sorghum | |
dc.subject | charcoal rot | |
dc.subject | MinION | |
dc.subject | Oxford Nanopore | |
dc.title | Draft Genome Resource for Macrophomina phaseolina Associated With Charcoal Rot in Sorghum | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 33 | |
dc.date.issued | 2020 | |
local.identifier.absfor | 060705 - Plant Physiology | |
local.identifier.ariespublication | a383154xPUB13237 | |
local.publisher.url | http://apsjournals.apsnet.org/loi/mpmi | |
local.type.status | Published Version | |
local.contributor.affiliation | Purushotham, Neeraj, University of Southern Queensland | |
local.contributor.affiliation | Jones, Ashley, College of Science, ANU | |
local.contributor.affiliation | Poudel, Barsha, University of Southern Queensland | |
local.contributor.affiliation | Nasim, Jamila, College of Science, ANU | |
local.contributor.affiliation | Adorada, Dante, University of Southern Queensland | |
local.contributor.affiliation | Sparks, Adam, University of Southern Queensland | |
local.contributor.affiliation | Schwessinger, Benjamin, College of Science, ANU | |
local.contributor.affiliation | Vaghefi, Niloofar, University of Southern Queensland | |
local.description.embargo | 2099-12-31 | |
local.bibliographicCitation.issue | 5 | |
local.bibliographicCitation.startpage | 724 | |
local.bibliographicCitation.lastpage | 726 | |
local.identifier.doi | 10.1094/MPMI-12-19-0356-A | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2022-08-21T08:16:11Z | |
local.identifier.scopusID | 2-s2.0-85084130419 | |
local.identifier.thomsonID | WOS:000531759000005 | |
Collections | ANU Research Publications |
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File | Description | Size | Format | Image |
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01_Purushotham_Draft_Genome_Resource_for_2020.pdf | 439.94 kB | Adobe PDF | Request a copy |
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