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Draft Genome Resource for Macrophomina phaseolina Associated With Charcoal Rot in Sorghum

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Purushotham, Neeraj; Jones, Ashley; Poudel, Barsha; Nasim, Jamila; Adorada, Dante; Sparks, Adam; Schwessinger, Benjamin; Vaghefi, Niloofar

Description

Macrophomina phaseolina is a soil-borne phytopathogenic fungus that causes charcoal rot in several plant species, including sorghum. We constructed a draft genome of M. phaseolina isolate BRIP 70780a from sorghum, using long-read native DNA from MinION sequencing, which was error-corrected using short-read Illumina MiSeq reads. The draft genome, consisting of 22 contigs with an N50 of 4,257,441 bp, 99.3% complete benchmarking universal single-copy orthologs, and 14,471 genes, is a valuable...[Show more]

dc.contributor.authorPurushotham, Neeraj
dc.contributor.authorJones, Ashley
dc.contributor.authorPoudel, Barsha
dc.contributor.authorNasim, Jamila
dc.contributor.authorAdorada, Dante
dc.contributor.authorSparks, Adam
dc.contributor.authorSchwessinger, Benjamin
dc.contributor.authorVaghefi, Niloofar
dc.date.accessioned2021-02-22T23:04:36Z
dc.identifier.issn0894-0282
dc.identifier.urihttp://hdl.handle.net/1885/224127
dc.description.abstractMacrophomina phaseolina is a soil-borne phytopathogenic fungus that causes charcoal rot in several plant species, including sorghum. We constructed a draft genome of M. phaseolina isolate BRIP 70780a from sorghum, using long-read native DNA from MinION sequencing, which was error-corrected using short-read Illumina MiSeq reads. The draft genome, consisting of 22 contigs with an N50 of 4,257,441 bp, 99.3% complete benchmarking universal single-copy orthologs, and 14,471 genes, is a valuable resource to aid future studies in population genomics and molecular diagnostic marker development for rapid detection of the pathogen
dc.description.sponsorshipThis research was funded and supported by the Grains Research and Development Corporation (projects GRDC 9176684, DAQ00194, and DAQ00186), Australia.
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherAmerican Phytopathological Society
dc.rights© 2020 The American Phytopathological Society
dc.sourceMolecular Plant-Microbe Interactions (MPMI)
dc.subjectMacrophomina phaseolina
dc.subjectSorghum
dc.subjectcharcoal rot
dc.subjectMinION
dc.subjectOxford Nanopore
dc.titleDraft Genome Resource for Macrophomina phaseolina Associated With Charcoal Rot in Sorghum
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume33
dc.date.issued2020
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationa383154xPUB13237
local.publisher.urlhttp://apsjournals.apsnet.org/loi/mpmi
local.type.statusPublished Version
local.contributor.affiliationPurushotham, Neeraj, University of Southern Queensland
local.contributor.affiliationJones, Ashley, College of Science, ANU
local.contributor.affiliationPoudel, Barsha, University of Southern Queensland
local.contributor.affiliationNasim, Jamila, College of Science, ANU
local.contributor.affiliationAdorada, Dante, University of Southern Queensland
local.contributor.affiliationSparks, Adam, University of Southern Queensland
local.contributor.affiliationSchwessinger, Benjamin, College of Science, ANU
local.contributor.affiliationVaghefi, Niloofar, University of Southern Queensland
local.description.embargo2099-12-31
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage724
local.bibliographicCitation.lastpage726
local.identifier.doi10.1094/MPMI-12-19-0356-A
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2022-08-21T08:16:11Z
local.identifier.scopusID2-s2.0-85084130419
local.identifier.thomsonIDWOS:000531759000005
CollectionsANU Research Publications

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