Skip navigation
Skip navigation

Nanometer-Scale Distance Measurements in Proteins Using Gd 3+ Spin Labeling

Potapov, Alexey; Yagi, Hiromasa; Jergic, Slobodan; Dixon, Nicholas Edward; Goldfarb, Daniella; Otting, Gottfried; Huber, Thomas


Methods for measuring nanometer-scale distances between specific sites in proteins are essential for analysis of their structure and function. In this work we introduce Gd3+ spin labeling for nanometer-range distance measurements in proteins by high-field pulse electron paramagnetic resonance (EPR). To evaluate the performance of such measurements, we carried out four-pulse double-electron electron resonance (DEER) measurements on two proteins, p75ICD and τC14, labeled at strategically selected...[Show more]

CollectionsANU Research Publications
Date published: 2010
Type: Journal article
Source: Journal of the American Chemical Society
DOI: 10.1021/ja1015662


File Description SizeFormat Image
01_Potapov_Nanometer-Scale_Distance_2010.pdf2.07 MBAdobe PDF    Request a copy

Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  23 August 2018/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator