Nanometer-Scale Distance Measurements in Proteins Using Gd 3+ Spin Labeling
Methods for measuring nanometer-scale distances between specific sites in proteins are essential for analysis of their structure and function. In this work we introduce Gd3+ spin labeling for nanometer-range distance measurements in proteins by high-field pulse electron paramagnetic resonance (EPR). To evaluate the performance of such measurements, we carried out four-pulse double-electron electron resonance (DEER) measurements on two proteins, p75ICD and τC14, labeled at strategically selected...[Show more]
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|Source:||Journal of the American Chemical Society|
|01_Potapov_Nanometer-Scale_Distance_2010.pdf||2.07 MB||Adobe PDF||Request a copy|
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