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Nanometer-Scale Distance Measurements in Proteins Using Gd 3+ Spin Labeling

Potapov, Alexey; Yagi, Hiromasa; Huber, Thomas; Jergic, Slobodan; Dixon, Nicholas Edward; Goldfarb, Daniella; Otting, Gottfried

Description

Methods for measuring nanometer-scale distances between specific sites in proteins are essential for analysis of their structure and function. In this work we introduce Gd3+ spin labeling for nanometer-range distance measurements in proteins by high-field pulse electron paramagnetic resonance (EPR). To evaluate the performance of such measurements, we carried out four-pulse double-electron electron resonance (DEER) measurements on two proteins, p75ICD and τC14, labeled at strategically selected...[Show more]

CollectionsANU Research Publications
Date published: 2010
Type: Journal article
URI: http://hdl.handle.net/1885/22106
Source: Journal of the American Chemical Society
DOI: 10.1021/ja1015662

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