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Selective Translation Complex Profiling Reveals Staged Initiation and Co-translational Assembly of Initiation Factor Complexes

Wagner, Susan; Herrmannová, Anna; Hronová, Vladislava; Gunišová, Stanislava; Sen, Neelam D; Hannan, Ross; Hinnebusch, Alan Gerard; Shirokikh, Nikolay; Preiss, Thomas; Valasek, Leos Shivaya


Translational control targeting the initiation phase is central to the regulation of gene expression. Understanding all of its aspects requires substantial technological advancements. Here we modified yeast translation complex profile sequencing (TCP-seq), related to ribosome profiling, and adapted it for mammalian cells. Human TCP-seq, capable of capturing footprints of 40S subunits (40Ss) in addition to 80S ribosomes (80Ss), revealed that mammalian and yeast 40Ss distribute similarly across...[Show more]

CollectionsANU Research Publications
Date published: 2020
Type: Journal article
Source: Molecular Cell
DOI: 10.1016/j.molcel.2020.06.004
Access Rights: Open Access


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