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A GDSL Esterase/Lipase Catalyzes the Esterification of Lutein in Bread Wheat

Watkins, Jacinta; Li, Ming; McQuinn, Ryan; Chan, Kai Xun; McFarlane, Heather E.; Ermakova, Maria; Furbank, Robert; Mares, Daryl; Dong, Chongmei; Chalmers, Kenneth J.; Sharp, Peter; Mather, Diane E.; Pogson, Barry

Description

Xanthophylls are a class of carotenoids that are important micronutrients for humans. They are often found esterified with fatty acids in fruits, vegetables, and certain grains, including bread wheat (Triticum aestivum). Esterification promotes the sequestration and accumulation of carotenoids, thereby enhancing stability, particularly in tissues such as in harvested wheat grain. Here, we report on a plant xanthophyll acyltransferase (XAT) that is both necessary and sufficient for xanthophyll...[Show more]

dc.contributor.authorWatkins, Jacinta
dc.contributor.authorLi, Ming
dc.contributor.authorMcQuinn, Ryan
dc.contributor.authorChan, Kai Xun
dc.contributor.authorMcFarlane, Heather E.
dc.contributor.authorErmakova, Maria
dc.contributor.authorFurbank, Robert
dc.contributor.authorMares, Daryl
dc.contributor.authorDong, Chongmei
dc.contributor.authorChalmers, Kenneth J.
dc.contributor.authorSharp, Peter
dc.contributor.authorMather, Diane E.
dc.contributor.authorPogson, Barry
dc.date.accessioned2021-01-10T23:14:58Z
dc.identifier.issn1040-4651
dc.identifier.urihttp://hdl.handle.net/1885/219256
dc.description.abstractXanthophylls are a class of carotenoids that are important micronutrients for humans. They are often found esterified with fatty acids in fruits, vegetables, and certain grains, including bread wheat (Triticum aestivum). Esterification promotes the sequestration and accumulation of carotenoids, thereby enhancing stability, particularly in tissues such as in harvested wheat grain. Here, we report on a plant xanthophyll acyltransferase (XAT) that is both necessary and sufficient for xanthophyll esterification in bread wheat grain. XAT contains a canonical Gly-Asp-Ser-Leu (GDSL) motif and is encoded by a member of the GDSL esterase/lipase gene family. Genetic evidence from allelic variants of wheat and transgenic rice (Oryza sativa) calli demonstrated that XAT catalyzes the formation of xanthophyll esters. XAT has broad substrate specificity and can esterify lutein, β-cryptoxanthin, and zeaxanthin using multiple acyl donors, yet it has a preference for triacylglycerides, indicating that the enzyme acts via transesterification. A conserved amino acid, Ser-37, is required for activity. Despite xanthophylls being synthesized in plastids, XAT accumulated in the apoplast. Based on analysis of substrate preferences and xanthophyll ester formation in vitro and in vivo using xanthophyll-accumulating rice callus, we propose that disintegration of the cellular structure during wheat grain desiccation facilitates access to lutein-promoting transesterification.
dc.description.sponsorshipThis research was funded by the Grains Research and Development Corporation (UA00102), Australian Research Council (ARC) Centre of Excellence in Plant Energy Biology (CE140100008), and the ARC Centre of Excellence in Translational Photosynthesis (CE1401000015). Support was also provided by ARC Discovery Early Career Reasearch Award (DE170100054 to H.E.M.), by the Research Foundation – Flanders (postdoctoral fellowship 12N4818N to K.X.C.), and by an Australian Research Training Program (RTP) scholarship to J.L.W.
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherAmerican Society of Plant Biologists
dc.rights© 2019 ASPB
dc.sourceThe Plant Cell
dc.titleA GDSL Esterase/Lipase Catalyzes the Esterification of Lutein in Bread Wheat
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume31
dc.date.issued2019
local.identifier.absfor060702 - Plant Cell and Molecular Biology
local.identifier.absfor070303 - Crop and Pasture Biochemistry and Physiology
local.identifier.absfor070305 - Crop and Pasture Improvement (Selection and Breeding)
local.identifier.ariespublicationa383154xPUB10930
local.publisher.urlhttp://www.plantcell.org/
local.type.statusPublished Version
local.contributor.affiliationWatkins, Jacinta, College of Science, ANU
local.contributor.affiliationLi, Ming, University of Adelaide
local.contributor.affiliationMcQuinn, Ryan, College of Science, ANU
local.contributor.affiliationChan, Kai Xun, Ghent University
local.contributor.affiliationMcFarlane, Heather E., University of Melbourne
local.contributor.affiliationErmakova, Maria, College of Science, ANU
local.contributor.affiliationFurbank, Robert, College of Science, ANU
local.contributor.affiliationMares, Daryl, University of Adelaide
local.contributor.affiliationDong, Chongmei , The University of Sydney
local.contributor.affiliationChalmers, Kenneth J. , University of Adelaide
local.contributor.affiliationSharp, Peter , University of Sydney
local.contributor.affiliationMather, Diane E., University of Adelaide
local.contributor.affiliationPogson, Barry, College of Science, ANU
local.description.embargo2099-12-31
dc.relationhttp://purl.org/au-research/grants/arc/CE140100008
dc.relationhttp://purl.org/au-research/grants/arc/DE170100054
local.bibliographicCitation.issue12
local.bibliographicCitation.startpage3092
local.bibliographicCitation.lastpage3112
local.identifier.doi10.1105/tpc.19.00272
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
local.identifier.absseo820507 - Wheat
dc.date.updated2020-11-02T04:16:45Z
CollectionsANU Research Publications

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