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Site-specific labelling of proteins with a rigid lanthanide-binding tag

Su, Xun-Cheng; Dixon, Nicholas; Otting, Gottfried; Huber, Thomas


This paper describes a generic method for the site-specific attachment of lanthanide complexes to proteins through a disulfide bond. The method is demonstrated by the attachment of a lanthanide-binding peptide tag to the single cysteine residue present in the N-terminal DNA-binding domain of the Escherichia coli arginine repressor. Complexes with Y3+, Tb3+, Dy3+, Ho3+, Er3+, Tm3+ and Yb3+ ions were formed and analysed by NMR spectroscopy. Large pseudocontact shifts and residual dipolar...[Show more]

CollectionsANU Research Publications
Date published: 2006
Type: Journal article
Source: ChemBioChem
DOI: 10.1002/cbic.200600142


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